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. 2023 Nov 25;28(23):7773.
doi: 10.3390/molecules28237773.

The Characterization and Study of Antibacterial, Free Radical Scavenging, and Anticancer Potential of Livistona chinensis-Mediated Silver Nanoparticles

Aroona Saleem  1 , Sikander Ali  1 , Muhammad Nauman Aftab  1 , Ashwag Shami  2 , Fatimah A Al-Saeed  3 , Bilal Mustafa  4 , Bilal Ahamad Paray  5
Affiliations

The Characterization and Study of Antibacterial, Free Radical Scavenging, and Anticancer Potential of Livistona chinensis-Mediated Silver Nanoparticles

Aroona Saleem et al. Molecules. .

Abstract

In the present research, Livistona chinensis leaf extracts were utilized as reductants to bio-fabricate silver nanoparticles (LC-AgNPs) and this was followed by the evaluation of their antioxidant, antibacterial, and anticancer potential. Multiple parameters were optimized for the formation and fidelity of LC-AgNPs. The color shift of the reaction mixture from yellow to dark brown confirmed the LC-AgNPs formation. UV/VIS spectroscopy exhibited a surface plasmon resonance (SPR) band at 436 nm. The Fourier transform infrared (FTIR) spectroscopy spectrum depicted phytochemicals in the plant extract acting as bio-reducers for LC-AgNPs synthesis. The XRD pattern confirmed the presence of LC-AgNPs by showing peaks corresponding to 2θ angle at 8.24° (111), 38.16° (200), 44.20° (220), and 64.72° (311). Zetasizer analysis exhibited size distribution by intensity of LC-AgNPs with a mean value of 255.7 d. nm. Moreover, the zeta potential indicated that the AgNPs synthesized were stable. The irregular shape of LC-AgNPs with a mean average of 38.46 ± 0.26 nm was found by scanning electron microscopy. Furthermore, the antioxidant potential of LC-AgNPs was examined using a DPPH assay and was calculated to be higher in LC-AgNPs than in leaf extracts. The calculated IC50 values of the LC-AgNPs and plant extract are 85.01 ± 0.17 and 209.44 ± 0.24, respectively. The antibacterial activity of LC-AgNPs was investigated against Escherichia coli, Pseudomonas aeruginosa, and Bacillus subtilis as well as Staphylococcus aureus, and maximum potential was observed after 24 h against P. aeruginosa. Moreover, LC-AgNPs exhibited maximum anticancer potential against TPC1 cell lines compared to the plant extract. The findings suggested that LC-AgNPs could be used as antioxidant, antibacterial, and anticancer agents for the cure of free-radical-oriented bacterial and oncogenic diseases.

Keywords: AgNPs; DPPH; Livistona chinensis; green synthesis; reactive oxygen species; thyroid cancer cells.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
UV–visible spectrums of optimization conditions for efficient leaf extracts: (a) different solvents used for leaf extracts, (b) different concentrations of leaf extracts, (c) dynamic extraction’s effect on leaf extracts, and (d) different temperatures for leaf extractions.
Figure 2
Figure 2
Metallurgical microscopy of L. chinensis leaf particles.
Figure 3
Figure 3
UV/VIS spectroscopy of plant extract and LC-AgNPs in comparison.
Figure 4
Figure 4
FTIR spectroscopy of LC-AgNPs and the plant extract.
Figure 5
Figure 5
X-Ray diffraction analysis of LC-AgNPs.
Figure 6
Figure 6
Zeta potential distribution of LC-AgNPs.
Figure 7
Figure 7
Size distribution by intensity of LC-AgNPs.
Figure 8
Figure 8
(a) SEM images of LC-AgNPs (b) Size distribution histogram of LC-AgNPs.
Figure 8
Figure 8
(a) SEM images of LC-AgNPs (b) Size distribution histogram of LC-AgNPs.
Figure 9
Figure 9
Bar graph depicting DPPH radical scavenging activity of L. chinensis extract, LC- AgNPs, and ascorbic acid.
Figure 10
Figure 10
Agar well diffusion assay against E. coli, S. aureus, B. subtilis, and P. aeruginosa after 24 h.
Figure 11
Figure 11
(a) TPC1 cell lines under the influence of plant extract, free AgNPs, and LC-AgNPs. (b) Bar graph depicting 10 and 15% dilutions of plant extract, free AgNPs, and LC-AgNPs in comparison with control during 30 min exposure in a TPC1 cell line.
Figure 12
Figure 12
Synthesis of LC-AgNPs by using leaf extracts of L. chinensis.
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