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. 2023 Sep;16(Suppl 2):190-194.
doi: 10.5005/jp-journals-10005-2631.

Effect of Passion Fruit Juice in Removal of Smear Layer in Root Canal of Ex Vivo Human Teeth: A Scanning Electron Microscopic Study

Vinothini Venkatachalamoorthi  1 , Prathima G Shivashankarappa  2 , Sanguida Adimoulame  2 , Kayalvizhi Gurusamy  3 , Kavitha Muthukrishnan  4 , Ezhumalai Govindan  5
Affiliations

Effect of Passion Fruit Juice in Removal of Smear Layer in Root Canal of Ex Vivo Human Teeth: A Scanning Electron Microscopic Study

Vinothini Venkatachalamoorthi et al. Int J Clin Pediatr Dent. 2023 Sep.

Abstract

Context: The smear layer produced during the instrumentation of the root canal contains both organic and inorganic components, and it is recommended to be removed as it has a mixture of bacteria and their byproducts. Irrigants like sodium hypochlorite (NaOCl), ethylenediaminetetraacetic acid (EDTA), endodontic irrigant, citric acid, etc., possess the ability to remove the smear layer. Considering the antimicrobial effect, antiinflammatory, cavity disinfectant, nontoxicity, better taste, and aroma of herbal products, a novel approach has been introduced in the field of endodontics.

Aims: To assess the efficacy of passion fruit juice in removing the smear layer and its erosive potential in root canals after instrumentation by scanning electron microscopy.

Materials and methods: About 35 single-rooted premolars were divided into five groups-30% passion fruit extract (PFE), 17% EDTA, 17% EDTA + 5.25% NaOCl, 30% PFE + 17% EDTA, and saline. Following irrigation with the above irrigants, each tooth was split into two halves and examined and scored for smear layer removal (modified Torabinejad's criteria) using a scanning electron microscope.

Statistical analysis: Overall comparison of the irrigant's action on removing the smear layer and their erosive potential was done using the Kruskal-Wallis test, and intergroup comparison of the irrigant action was done using the Mann-Whitney U test. All the statistical analyses were set with a significance level of p < 0.05.

Results: A total of 30% PFE produced less erosion and statistically significant smear layer removal in the coronal and middle third of the root. When combined with 17% EDTA, it effectively removed the smear layer in all three-thirds of the root. Around 30% PFE + 17% EDTA is less erosive when compared to 17% EDTA + 5.25% NaOCl.

Conclusion: This study revealed that 30% PFE and 30% PFE + 17% EDTA are promising irrigants as root canal disinfectants in endodontics.

How to cite this article: Venkatachalamoorthi V, Shivashankarappa PG, Adimoulame S, et al. Effect of Passion Fruit Juice in Removal of Smear Layer in Root Canal of Ex Vivo Human Teeth: A Scanning Electron Microscopic Study. Int J Clin Pediatr Dent 2023;16(S-2):S190-S194.

Keywords: Erosion; Ethylenediaminetetraacetic acid; Passion fruit extract; Smear layer; Sodium hypochlorite.

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Conflict of interest statement

Source of support: Nil Conflict of interest: None Conflict of interest: Dr Prathima G Shivashankarappa is associated as the National Editorial Board member of this journal and this manuscript

Figures

Fig. 1
Fig. 1
Preparation of alcoholic extract of passion fruit, passion whole fruit and pulp
Fig. 2
Fig. 2
Decoronation of the samples and root apex covered with sticky wax
Fig. 3
Fig. 3
Working length estimation and irrigation with saline
Fig. 4
Fig. 4
Samples after sputtering on stubs
Fig. 5
Fig. 5
Representative SEM images for smear layer removal and erosion of dentinal tubules at the coronal, middle, and apical third of the sample
Fig. 6
Fig. 6
Mean erosion scores for each group at each region
See this image and copyright information in PMC

References

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