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. 2024 Jan;177(1):113-115.
doi: 10.7326/L23-0253. Epub 2023 Dec 12.

Replication-Competent Virus Detected in Blood of a Fatal COVID-19 Case

Andrew Platt  1 , Manmeet Singh  2 , Sydney Stein  1 , Shahabuddin Soherwardi  3 , Emmie de Wit  2 , Daniel S Chertow  1
Affiliations

Replication-Competent Virus Detected in Blood of a Fatal COVID-19 Case

Andrew Platt et al. Ann Intern Med. 2024 Jan.
No abstract available

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Conflict of interest statement

Disclosures: Disclosures can be viewed at www.acponline.org/authors/icmje/ConflictOfInterestForms.do?msNum=L23-0253.

Figures

Figure.
Figure.. Sequencing results from primary samples and cell culture supernatants.
NSP = nonstructural protein; UTR = untranslated region. Top. Representative schematic of the SARS-CoV-2 genome. Due to low input, a full-length contig could not be generated for the original plasma and lung samples. The location of the gaps in sequences is represented by green bars with diagonal lines for the plasma sample and by solid purple bars for the lung sample. Bottom. Synonymous and nonsynonymous mutations present in the SARS-CoV-2 sequence from the plasma, cell culture isolate from plasma, lung, cell culture isolate from the lung, and cell culture isolate from the heart as obtained by short-read sequencing and in reference to the SARS-CoV-2 2019-nCoV/USA-WA1/2020 genome (MN985325.1). Libraries were generated using the Illumina Ligation Prep kit sequenced on a MiSeq instrument (Illumina). The autopsy plasma sample had hybrid capture virus enrichment using myBaits Expert Virus SARS-CoV-2 panel (Arbor Biosciences) before sequencing. Values represent alternative variant percentage reads. * Additional mutations present in cell culture isolate from plasma samples.
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