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. 2024 Apr;71(2):387-401.
doi: 10.1002/bab.2547. Epub 2023 Dec 11.

Resveratrol-loaded decellularized ovine pericardium: ECM introduced for tissue engineering

Mozafar Khazaei  1   2 , Morteza Alizadeh  3 , Leila Rezakhani  1   2
Affiliations

Resveratrol-loaded decellularized ovine pericardium: ECM introduced for tissue engineering

Mozafar Khazaei et al. Biotechnol Appl Biochem. 2024 Apr.

Abstract

An ideal scaffold for skin tissue engineering should have a suitable potential for antibacterial activity, no hemolysis, sufficient porosity for air exchange, water retention capacity, and a suitable swelling rate to maintain tissue moisture. Considering this issue, our study used decellularized ovine pericardial tissue's extracellular matrix (ECM). These scaffolds were decellularized with sodium dodecyl sulfate (SDS) and sodium deoxycholate (SD) detergents along with vacuum methods. Following imaging with scanning electron microscopy (SEM), analysis of the mechanical properties, and the measurement of the amount of DNA, collagen, and glycosaminoglycan (GAG), our study observed that the three-dimensional (3D) structure of ECM was largely preserved. Resveratrol (RES) 400 µg/µL was loaded into the above scaffold, and analysis revealed that scaffolds containing RES and with vacuum reported higher antibacterial properties, a higher swelling rate, and increased water retention capacity. The biocompatibility and hemocompatibility properties of the above scaffolds also reported a significant difference between methods of decellularization.

Keywords: decellularized tissue; resveratrol; tissue engineering.

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References

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