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. 2024 Feb;69(2):437-452.
doi: 10.1007/s10620-023-08206-7. Epub 2023 Dec 12.

CYP1B1-AS1 Delays the Malignant Progression of Colorectal Cancer by Binding with NOP58

Zhao Wu  1   2 , Fei Cheng  1 , Lebin Yuan  1   2 , Xiaodong Li  1   2 , Zhigang Li  1   2 , Zeyu Huang  1   2 , Shengping Mao  1   2 , Xing Chen  1   2 , Jiwei Wang  3 , Bin Lai  1 , Wei Shen  4
Affiliations

CYP1B1-AS1 Delays the Malignant Progression of Colorectal Cancer by Binding with NOP58

Zhao Wu et al. Dig Dis Sci. 2024 Feb.

Abstract

Background: Colorectal cancer (CRC) is a prevalent type of gastrointestinal cancer, and its poor prognosis is mainly attributed to the occurrence of invasion and metastasis. CYP1B1-AS1, as non-coding RNA, plays an important role in tumorigenesis and progression. However, the mechanism by which CYP1B1-AS1 acts in CRC is not yet understood.

Aims: The objective of this study was to investigate how CYP1B1-AS1 contributes to the development of CRC, and provide a base for CRC diagnosis and treatment.

Methods: RT-qPCR was used to detect the expression level of CYP1B1-AS1 in CRC and adjacent tissues. CCK-8, Edu, scratch healing, and transwell experiments were used to detect the changes of proliferation, migration, and invasion ability of CRC cells after overexpression or knockdown of CYP1B1-AS1 respectively. The RNA binding protein NOP58 combined with CYP1B1-AS1 was verified by RIP and RNA Pull-down experiments. Functional recovery experiments validated the interaction between CYP1B1-AS1 and NOP58 in CRC cells. The changes of EMT-related proteins were detected by Western blot, and the half-life of transcription factor SNAIL mRNA were detected by RT-qPCR after overexpression or knockdown of NOP58.

Results: CYP1B1-AS1 was found to be significantly downregulated in CRC compared to adjacent noncancerous tissues. Experiments conducted in vitro and in vivo confirmed that upregulation of CYP1B1-AS1 significantly inhibited the proliferation, migration, and invasion of CRC cells. In addition, CYP1B1-AS1 can directly bind to NOP58 and negatively regulate NOP58. The effect of overexpression CYP1B1-AS1 was reversed by NOP58 overexpression. NOP58 regulates the EMT process of CRC cells by affecting the stability of EMT-related transcription factor SNAIL mRNA, and then affects the progress of CRC.

Conclusion: This research proves that CYP1B1-AS1 can inhibit the occurrence of EMT in CRC by binding with NOP58, thus delaying the progress of CRC. This finding indicates that CYP1B1-AS1 may be a novel biomarker to improve the diagnosis and treatment of CRC.

Keywords: CYP1B1-AS1; Colorectal cancer; Long noncoding RNA; NOP58; RNA-binding protein.

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References

    1. Sung H, Ferlay J, Siegel RL et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: Cancer J Clin 2021;71:209–249. - PubMed
    1. Liang TJ, Wang HX, Zheng YY et al. APC hypermethylation for early diagnosis of colorectal cancer: a meta-analysis and literature review. Oncotarget 2017;8:46468–46479. - DOI - PubMed - PMC
    1. Miller KD, Nogueira L, Mariotto AB et al. Cancer treatment and survivorship statistics, 2019. CA: Cancer J Clin 2019;69:363–85. - PubMed
    1. Ponting CP, Oliver PL, Reik W. Evolution and functions of long noncoding RNAs. Cell 2009;136:629–641. - DOI - PubMed
    1. Gibb EA, Brown CJ, Lam WL. The functional role of long non-coding RNA in human carcinomas. Mol cancer 2011;10:38. - DOI - PubMed - PMC

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