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The conformational state of a structural protein in bacteria can vary, depending on the concentration level of potassium ions or the nucleotide bound to it.
Keywords:
ATPase; Geobacillus stearothermophilus; actin-like MreB; biochemistry; chemical biology; crystal structure; electron microscopy; polymerization.
(A) In the absence of the protein MreB, cell-like structures called liposomes are spherical. (B) In the presence of ATP (blue circles) but not ADP or AMPPNP (red and orange circles), MreB filaments induce a negative membrane curvature on liposomes when salt concentrations are low. (C) When salt concentrations are high, MreB filaments induce a tubular shape in the liposome.
Mao W, Renner LD, Cornilleau C, Li de la Sierra-Gallay I, Afensiss S, Benlamara S, Ah-Seng Y, Van Tilbeurgh H, Nessler S, Bertin A, Chastanet A, Carballido-Lopez R.Mao W, et al.Elife. 2023 Oct 11;12:e84505. doi: 10.7554/eLife.84505.Elife. 2023.PMID: 37818717Free PMC article.
References
Bean GJ, Amann KJ. Polymerization properties of the Thermotoga maritima actin MreB: roles of temperature, nucleotides, and ions. Biochemistry. 2008;47:826–835. doi: 10.1021/bi701538e.
-
DOI
-
PMC
-
PubMed
Bork P, Sander C, Valencia A. An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin, and hsp70 heat shock proteins. PNAS. 1992;89:7290–7294. doi: 10.1073/pnas.89.16.7290.
-
DOI
-
PMC
-
PubMed
Domínguez-Escobar J, Chastanet A, Crevenna AH, Fromion V, Wedlich-Söldner R, Carballido-López R. Processive movement of MreB-associated cell wall biosynthetic complexes in bacteria. Science. 2011;333:225–228. doi: 10.1126/science.1203466.
-
DOI
-
PubMed
Esue O, Cordero M, Wirtz D, Tseng Y. The assembly of MreB, a prokaryotic homolog of actin. The Journal of Biological Chemistry. 2005;280:2628–2635. doi: 10.1074/jbc.M410298200.
-
DOI
-
PubMed
Garner EC, Bernard R, Wang W, Zhuang X, Rudner DZ, Mitchison T. Coupled, circumferential motions of the cell wall synthesis machinery and MreB filaments in B. subtilis. Science. 2011;333:222–225. doi: 10.1126/science.1203285.
-
DOI
-
PMC
-
PubMed