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. 2023 Nov;176(1):42-49.
doi: 10.1007/s10517-023-05964-2. Epub 2023 Dec 13.

Morphopathological Effect of Ferrihydrite Nanoparticles in an Experimental Model of Toxic Hemolytic Anemia

A V Kireeva  1 , O A Kolenchukova  2   3 , M V Loshkareva  4 , E A Biryukova  2 , V P Ladygina  4 , S V Stolyar  4   3
Affiliations

Morphopathological Effect of Ferrihydrite Nanoparticles in an Experimental Model of Toxic Hemolytic Anemia

A V Kireeva et al. Bull Exp Biol Med. 2023 Nov.

Abstract

We studied the effect of biogenic ferrihydrite nanoparticles synthesized as a result of the culturing of Klebsiella oxytoca on Wistar rats with experimental toxic hemolytic anemia. The pathology was simulated by single intraperitoneal injection of phenylhydrazine hydrochloride. On day 4, the functional parameters of erythrocytes in rats corresponded to the state of toxic hemolytic anemia. It is shown that ferrihydrite nanoparticles suspension has chronic toxicity and causes morphological changes in organs (mainly in the spleen), which are characterized by accumulation of nanoparticles. Administration of phenylhydrazine induced systemic vascular damage and the formation of extramedullary hematopoietic foci, which indicated a compensatory activation in hematopoiesis in the liver and spleen. Injection of nanoparticles reduced discirculatory and necrotic changes in the kidneys.

Keywords: clinical blood parameters; iron oxide nanoparticles; pathomorphology; rats; toxic hemolytic anemia.

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