Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Mar;17(1):e20418.
doi: 10.1002/tpg2.20418. Epub 2023 Dec 13.

Identification of a new Rsg1 allele conferring resistance to multiple greenbug biotypes from barley accessions PI 499276 and PI 566459

Xiangyang Xu  1 , Dolores Mornhinweg  1 , Guihua Bai  2 , Genqiao Li  1 , Ruolin Bian  3 , Amy Bernardo  2 , J Scott Armstrong  1
Affiliations
Free article

Identification of a new Rsg1 allele conferring resistance to multiple greenbug biotypes from barley accessions PI 499276 and PI 566459

Xiangyang Xu et al. Plant Genome. 2024 Mar.
Free article

Abstract

Greenbug [Schizaphis graminum (Rondani)] is a major insect pest that significantly affects barley production worldwide. The identification of novel greenbug resistance genes is crucial for sustainable barley production and global food security. To identify greenbug resistance genes from a US breeding line PI 499276 and a Chinese cultivar PI 566459, two F6:7 recombinant inbred line (RIL) populations developed from crosses Weskan × PI 499276 and Weskan × PI 566459 were phenotyped for responses to greenbug biotype E and genotyped using genotyping-by-sequencing (GBS). Linkage analysis using single nucleotide polymorphism and kompetitive allele-specific polymorphism (KASP) markers delimited the greenbug resistance genes from PI 499276 and PI 566459 to a 1.2 Mb genomic region between 666.5 and 667.7 Mb on the long arm of chromosome 3H in the Morex Hordeum vulgare r1 reference sequence. Allelism tests based on responses of four F2 populations to greenbug biotype E indicated that the greenbug resistance gene in PI 499276 and PI 566459 is either allelic or very close to Rsg1. Given that PI 499276 and PI 566459 shared the same unique resistance pattern to a set of 14 greenbug biotypes, which is different from those of other Rsg1 alleles, they carry a new Rsg1 allele. The greenbug resistance genes in Post 90, PI 499276/PI 566459, and WBDC 336 were designated as Rsg1.a1, Rsg1.a2, and Rsg1.a3, respectively. KASP markers KASP-Rsg1a3-1, KASP-Rsg1a3-2, and KASP160 can be used to tag Rsg1.a2 in barley breeding.

PubMed Disclaimer

References

REFERENCES

    1. Aljaryian, R., & Kumar, L. (2016). Changing global risk of invading greenbug Schizaphis graminum under climate change. Crop Protection, 88, 137–148. https://doi.org/10.1016/j.cropro.2016.06.008
    1. Armstrong, J. S., Mornhinweg, D. W., Payton, M. E., & Puterka, G. J. (2016). The discovery of resistant sources of spring barley, Hordeum vulgare ssp. spontaneum, and unique greenbug biotypes. Journal of Economic Entomology, 109, 434–438. https://doi.org/10.1093/jee/tov320
    1. Bebber, D. P., Ramotowski, M. A., & Gurr, S. J. (2013). Crop pests and pathogens move polewards in a warming world. Nature Climate Change, 3, 985–988. https://doi.org/10.1038/nclimate1990
    1. Bradbury, P. J., Zhang, Z., Kroon, D. E., Casstevens, T. M., Ramdoss, Y., & Buckler, E. S. (2007). TASSEL: Software for association mapping of complex traits in diverse samples. Bioinformatics, 23, 2633–2635. https://doi.org/10.1093/bioinformatics/btm308
    1. Dubcovsky, J., Galvez, A. F., & Dvořák, J. (1994). Comparison of the genetic organization of the early salt‐stress‐response gene system in salt‐tolerant Lophopyrum elongatum and salt‐sensitive wheat. Theoretical and Applied Genetics, 87, 957–964. https://doi.org/10.1007/BF00225790

LinkOut - more resources