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. 2023 Nov 13;15(11):e48739.
doi: 10.7759/cureus.48739. eCollection 2023 Nov.

A Comparative Cytocompatibility Assessment of Leukocyte-Platelet-Rich Fibrin (L-PRF) and Injectable Platelet-Rich Fibrin (I-PRF) on the Pre-osteoblastic MG-63 Cell Line in Chronic Periodontitis Patients: An In Vitro Study

Sheryl Dolly A  1 , Ravishankar Pl  1 , Prem Blaisie Rajula  1 , Sai Sri Soury Geddam  1 , Lochini S  1 , Sindhujaa R  1 , Mohamed Rashik  1
Affiliations

A Comparative Cytocompatibility Assessment of Leukocyte-Platelet-Rich Fibrin (L-PRF) and Injectable Platelet-Rich Fibrin (I-PRF) on the Pre-osteoblastic MG-63 Cell Line in Chronic Periodontitis Patients: An In Vitro Study

Sheryl Dolly A et al. Cureus. .

Abstract

Aim The aim of the study is to assess the cellular viability of various concentrations of different platelet concentrates on pre-osteoblastic MG-63 cells. Materials and methods In this in-vitro experiment, blood samples from 21 individuals with chronic periodontitis were taken and centrifuged according to Choukroun and Miron's protocol to prepare L-PRF and I-PRF, respectively. The methyl thiazolyl tetrazolium (MTT) test was used to determine the viability of 0%, 1%, 2%, 4%, 8%, 10%, and 20% concentrations of L-PRF and I-PRF on MG-63 cells. Results The 20% L-PRF had the lowest percentage of cell viability (90.429±2.06), and the 1% I-PRF had the highest percentage (98.918±0.54), with no statistically significant difference (p>0.05). Conclusion According to the findings of the current study, both L-PRF and I-PRF provide favorable outcomes in terms of the viability of MG-63 cells in chronic periodontitis patients that may be utilized for regenerative purposes such as periodontal osseous defects and mucogingival surgeries. Incorporating these platelet concentrates with bone grafts results in enhanced regenerative outcomes.

Keywords: cell viability; cytocompatibility; i-prf; l-prf; mg-63 cells; platelet concentrates; regeneration.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Morphological evaluation using phase contrast microscopy: The biocompatibility of L-PRF and I-PRF, induced by both low (4%) and high (20%) concentrations, was evaluated in comparison with a control group on MG-63 osteoblast-like cells. This assessment was conducted using both phase contrast and fluorescent microscopy. Magnification: 20X.
L-PRF: Leukocyte-Platelet-Rich Fibrin, I-PRF: Injectable Platelet-Rich Fibrin.
Figure 2
Figure 2. Viability assessment of the osteoblastic cell line (MG-63) at various experimental concentrations in chronic periodontitis patients.
L-PRF: Leukocyte-Platelet-Rich Fibrin; I-PRF: Injectable Platelet-Rich Fibrin.
See this image and copyright information in PMC

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