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. 2023 Dec 16;72(4):433-442.
doi: 10.33073/pjm-2023-041. eCollection 2023 Dec 1.

Genomic Characterization of the Mycoparasite Pestalotiopsis sp. Strain cr013 from Cronartium ribicola

Jinde Yu  1 , Lei Kong  1 , Shichang Fan  1 , Mingjiao Li  1 , Jing Li  1
Affiliations

Genomic Characterization of the Mycoparasite Pestalotiopsis sp. Strain cr013 from Cronartium ribicola

Jinde Yu et al. Pol J Microbiol. .

Abstract

The Pestalotiopsis sp. strain cr013 is a mycoparasite of Cronartium ribicola, a potential biocontrol fungus for Armand pine (Pinus armandii) blister rust. A previous study showed that the strain cr013 has great potential to produce new compounds. However, there has been no report of the whole-genome sequence of the mycoparasite Pestalotiopsis sp. In this study, the BGISEQ-500 and Oxford Nanopore GridION X5 sequencing platforms were used to sequence the strain cr013 isolates and assemble the reads to obtain the complete genome. We first report the whole-genome information of the mycoparasite Pestalotiopsis sp. strain cr013 (GenBank accession number: JACFXT010000000, BioProject ID: PRJNA647543, BioSample ID: SAMN15589943), and the genomic components and gene functions related to the mycoparasitism process were analyzed. This study provides a theoretical basis for understanding the lifestyle strategy of the mycoparasite Pestalotiopsis sp. and reveals the mechanisms underlying secondary metabolite diversity in the strain cr013.

Keywords: Pestalotiopsis sp.; genome; lifestyle strategy; mycoparasitism; secondary metabolites.

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Conflict of interest statement

Conflict of interest

The authors do not report any financial or personal connections with other persons or organizations, which might negatively affect the contents of this publication and/or claim authorship rights to this publication.

Figures

Fig. 1.
Fig. 1.
Detection of the strain cr013 genome by electrophoresis. The concentration of agarose: 1%; voltage: 180 V; time: 36 min.; molecular weight standard name: M1: λ-Hind III digest (TaKaRa Bio, Inc., Japan), M2: D2000 (Tiangen Biotech(Beijing)Co., Ltd., China); sample volume: M1: 2.5 μl, M2: 5 μl.
Fig. 2.
Fig. 2.
Classification of the KEGG pathways of the cr013 strain.
Fig. 3.
Fig. 3.
COG functional classification of the cr013 strain.
Fig. 4.
Fig. 4.
GO functional annotation of the cr013 strain.
See this image and copyright information in PMC

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