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. 2024 Jan 17;13(1):e0108623.
doi: 10.1128/mra.01086-23. Epub 2023 Dec 15.

Genome sequences of 31 mycobacteriophages isolated on Mycobacterium smegmatis mc2155 at room temperature

Kirk R Anders  1 , Antonio Abeyta  1 , Christy C Andrade  1 , Carla Y Bonilla  1 , Amanda B Braley  1 , Alexandra G Bratt  1 , Kaya A Duncan  1 , Stephen G Hayes  1 , Ciara J Robinson  1 , Helen Smith-Flores  1 , Ann-Scott H Ettinger  1 , William F Ettinger  1 , Marta M Fay  1 , Joseph Haydock  1 , Sean K McKenzie  1 , Rebecca A Garlena  2 , Daniel A Russell  2 , Marianne K Poxleitner  1
Affiliations

Genome sequences of 31 mycobacteriophages isolated on Mycobacterium smegmatis mc2155 at room temperature

Kirk R Anders et al. Microbiol Resour Announc. .

Abstract

We report the genome sequences of 31 mycobacteriophages isolated on Mycobacterium smegmatis mc2155 at room temperature. The genomes add to the diversity of Clusters A, B, C, G, and K. Collectively, the genomes include 70 novel protein-coding genes that have no close relatives among the actinobacteriophages.

Keywords: bacteriophage.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig 1
Fig 1
Relationships among the 31 phages and all the phages of Clusters A, B, C, G, and K. Network trees of shared gene content are displayed, showing relationships among the phages within each cluster. The branch for each phage described in this report is marked with a red circle at its end, and its name and subcluster membership are indicated. To generate the trees, all protein-coding genes in the Actinobacteriophage Database (Actino_Draft, v.521) were grouped into families (phams) with Phamerator (14) based on amino acid sequence similarities. Each genome in a cluster was then scored according to the presence or absence of each pham, and the distance between each phage was computed by SplitsTree (v.4.19) (25), which generated an unrooted network representation of the relationships. The scale bars indicate 0.01 substitutions/site.
See this image and copyright information in PMC

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