Biochemical and Ultrastructural Changes in Wheat Plants during Drought Stress

Abstract

Drought severely slows down plant growth, decreases crop yield, and affects various physiological processes in plants. We examined four local bread wheat cultivars with different drought tolerance (drought-tolerant Zirva 85 and Murov 2 and drought-sensitive Aran and Gyzyl bughda cultivars). Leaves from seedlings of drought-tolerant plants demonstrated higher activity of antioxidant enzymes and lower levels of malondialdehyde and hydrogen peroxide. The content of soluble proteins in drought-exposed increased, possibly due to the stress-induced activation of gene expression and protein synthesis. Drought-exposed Zirva 85 plants exhibited an elevated activity of nitrogen and carbon metabolism enzymes. Ultrastructural analysis by transmission electron microscopy showed drought-induced damage to mesophyll cells and chloroplast membranes, although it was manifested less in the drought-tolerant cultivars. Comparative analysis of the activity of metabolic and antioxidant enzymes, as well as observed ultrastructural changes in drought-exposed plants revealed that the response to drought of seedlings was more pronounced in drought-tolerant cultivars. These findings can be used in further studies of drought stress in wheat plants under natural conditions.

Keywords: antioxidant enzymes; drought; metabolic enzymes; ultrastructural changes; wheat.