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. 2023 Sep;73(4):382-392.
doi: 10.1270/jsbbs.23017. Epub 2023 Sep 9.

Development and characterization of near-isogenic lines for brown planthopper resistance genes in the genetic background of japonica rice 'Sagabiyori'

Saw Bo Day Shar  1   2 , Cuong Dinh Nguyen  1   3 , Sachiyo Sanada-Morimura  4 , Hideshi Yasui  5 , Shao-Hui Zheng  6 , Daisuke Fujita  6
Affiliations

Development and characterization of near-isogenic lines for brown planthopper resistance genes in the genetic background of japonica rice 'Sagabiyori'

Saw Bo Day Shar et al. Breed Sci. 2023 Sep.

Abstract

The brown planthopper (BPH: Nilaparvata lugens Stål) is one of the most destructive insects in rice production. The use of host plant resistance has potential to reduce damage caused by BPH. The heat tolerance japonica rice 'Sagabiyori', with superior grain quality and high soluble starch in the stem, is highly susceptible to damage by BPH. Here, to enhance its BPH resistance, we developed seven near-isogenic lines (NILs) carrying BPH2, BPH17-ptb, BPH32, BPH3, BPH17, BPH20, and BPH21 through marker-assisted selection and evaluated resistance to two BPH populations. Most lines were more resistant to the Hadano-1966 BPH population than Sagabiyori but were less effective against the highly virulent Koshi-2013 population. Nevertheless, in antixenosis tests, Koshi-2013 settled less on all NILs than on Sagabiyori. In addition, adult mortality and the percentage of fresh weight loss of lines carrying BPH17 and BPH3 indicated that these lines have higher resistance to Koshi-2013 than Sagabiyori. Current study revealed that BPH resistance of Sagabiyori became stronger by transferring BPH3 and BPH17 genes. Thus, BPH3 and BPH17 might be valuable for breeding programs to enhance BPH resistance of high grain quality rice varieties with heat tolerance.

Keywords: BPH resistance gene; NILs; Nilaparvata lugens; marker-assisted selection.

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Figures

Fig. 1.
Fig. 1.
Breeding scheme for development of pre-NILs and NILs carrying BPH resistance genes in the Sagabiyori genetic background.
Fig. 2.
Fig. 2.
Graphical genotypes of NILs for BPH resistance genes in Sagabiyori genetic background. A. NILs for BPH17-ptb, BPH17, and BPH20 on chromosome 4. B. NILs for BPH3 and BPH32 on chromosome 6. C. NILs for BPH2 and BPH21 on chromosome 12. The map position of each marker is based on physical distance. Circles indicate the approximate position of resistance genes.
Fig. 3.
Fig. 3.
Results of screening test of pre-NILs carrying BPH resistance genes with two BPH populations: Damage score (DS) against A. Hadano-1966 and B. Koshi-2013 populations. Bars with the same letter are not significantly different at P < 0.05 by Tukey–Kramer test.
Fig. 4.
Fig. 4.
Antibiosis test of pre-NILs carrying BPH resistance genes against two BPH populations. A. and B. Adult mortality against A. Hadano-66 and B. Koshi-2013 populations. C. and D. Honeydew area of C. Hadano-66 and D. Koshi-2013 populations at 24 h after infestation. Bars with the same letter are not significantly different at P < 0.05 by Tukey–Kramer test.
Fig. 5.
Fig. 5.
Antixenosis test of pre-NILs carrying BPH resistance genes against two BPH populations. BPH settling against A. Hadano-1966 and B. Koshi-2013 populations. Asterisks indicate significant differences between pre-NILs and Sagabiyori: * P < 0.05, ** P < 0.01, *** P < 0.001 by t-test.
Fig. 6.
Fig. 6.
The percent of fresh plant weight loss on Pre-NILs carrying BPH resistance genes against Koshi-2013 BPH population through tolerance test. Bars with the same letter are not significantly different at P < 0.05 by Tukey–Kramer test.
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