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. 2024 Apr;104(6):3428-3436.
doi: 10.1002/jsfa.13228. Epub 2024 Jan 2.

Mixed ensiling plus nitrate destroy fiber structure of rape straw, increase degradation, and reduce methanogenesis through in vitro ruminal fermentation

Xuanxuan Pu  1   2 , Xiumin Zhang  1 , Siyu Yi  1 , Rong Wang  1 , Qiushuang Li  1 , Wanqian Zhang  1 , Jiajing Qu  1 , Jiabin Huo  1 , Bo Lin  3 , Bie Tan  2 , Zhiliang Tan  1 , Min Wang  1
Affiliations

Mixed ensiling plus nitrate destroy fiber structure of rape straw, increase degradation, and reduce methanogenesis through in vitro ruminal fermentation

Xuanxuan Pu et al. J Sci Food Agric. 2024 Apr.

Abstract

Background: Better utilization of rape straw can provide alternative strategies for sustainable ruminant and food production. The research reported here investigated changes in the carbohydrate composition of rape straw as a result of mixed ensiling with whole-crop corn or inoculated with nitrate, and the consequent effects on ruminal fermentation through in vitro batch culture. The three treatments included: rape straw and corn silage (RSTC), and ensiling treatment of rape straw with whole-crop corn (RSIC) or with calcium nitrate inoculation (RSICN).

Results: Ensiling treatment of rape straw and whole-crop corn or plus nitrate enriched lactic acid bacteria and lactate. The treatments broke the fiber surface connections of rape straw, leading to higher neutral detergent soluble (NDS) content and lower fiber content. Ensiling treatments led to greater (P < 0.05) dry matter degradation (DMD), molar proportions of propionate and butyrate, relative abundance of the phylum Bacteroidetes and genus Prevotella, and lower (P < 0.05) methane production in terms of g kg-1 DMD, molar proportions of acetate, and lower acetate to propionate ratio than the RSTC treatment. The RSICN treatment led to the lowest (P < 0.05) hydrogen concentration and methane production among the three treatments.

Conclusion: Ensiling treatments of rape straw and whole-crop corn destroy the micro-structure of rape straw, promote substrate degradation by enriching the phylum Bacteroidetes and the genus Prevotella, and decrease methane production by favoring propionate and butyrate production. Nitrate inoculation in the ensiling treatment of rape straw and whole-crop corn further decreases methane production without influencing substrate degradation by providing an additional hydrogen sink. © 2023 Society of Chemical Industry.

Keywords: bacterial community; fiber structure; methane production; rumen fermentation.

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References

REFERENCES

    1. Zhao X, Gong J, Zhou S, Ouyang K, Song X, Fu C et al., Effect of fungal treatments of rape straw on chemical composition and in vitro rumen fermentation characteristics. BioRes 10:622-637 (2014).
    1. Alexander BW, Gordon AH, Lomax JA and Chesson A, Composition and rumen degradability of straw from three varieties of oilseed rape before and after alkali, hydrothermal and oxidative treatment. J Sci Food Agr 41:1-15 (1987).
    1. Arvaniti E, Bjerre AB and Schmidt JE, Wet oxidation pretreatment of rape straw for ethanol production. Biomass Bioenergy 39:94-105 (2012).
    1. Zhao J, Tao X, Wang S, Li J and Shao T, Effect of sorbic acid and dual-purpose inoculations on the fermentation quality and aerobic stability of high dry matter rice straw silage. J Appl Microbiol 130:1456-1465 (2021).
    1. Ni K, Wang F, Zhu B, Yang J, Zhou G, Pan Y et al., Effects of LAB and molasses additives on the microbial community and fermentation quality of soybean silage. Bioresour Technol 238:706-715 (2017).

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