[Isolation of the glycoproteins of the Newcastle disease virus (NDV)]

Abstract

Two NDV glycoproteins--hemagglutinin-neuraminidase and F-protein--were separated from the remaining structural virus proteins through treatment of the virions with a 2 per cent triton-x-100 and chromatography on a Koh A-sepharose 4B column. A subsidiary ion-exchange chromatography on DEAE--Sephadex technique was employed to separate the two products and obtain them in a pure state. Biologically active NDV glycoproteins were obtained through solubilization of the virions with 10 per cent triton-x-100 at high ion strength, and the two glycoproteins were separated on a DEAE-bio gel column with the use of 0.01 M phosphate buffer of pH 7.2, containing 0.5 M NaCl.