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. 2023 Nov 21;9(12):e22691.
doi: 10.1016/j.heliyon.2023.e22691. eCollection 2023 Dec.

Optimization of cationic nanoparticles stabilized by poloxamer 188: A potential approach for improving the biological activity of Aloeperryi

Tahany Saleh Aldayel  1 , Mohamed M Badran  2   3 , Abdullah H Alomrani  2   3 , Nora A AlFaris  4 , Jozaa Z Altamimi  4 , Ali S Alqahtani  5 , Fahd A Nasr  6 , Safina Ghaffar  5 , Raha Orfali  5
Affiliations

Optimization of cationic nanoparticles stabilized by poloxamer 188: A potential approach for improving the biological activity of Aloeperryi

Tahany Saleh Aldayel et al. Heliyon. .

Abstract

Aloe perryi (AP) has gained considerable interest as a medicinal herb in various biological applications due to its rich phytochemical composition. However, the therapeutic benefits of AP could be potentiated by utilizing nanotechnology. Moreover, cationic solid lipid nanoparticles (CSLNs) possess remarkable characteristics that can greatly enrich a variety of biological uses. An optimization approach was used to achieve high-quality CSLNs to maximize the therapeutic efficacy of AP. Therefore, a factorial design was used to investigate the influence of various variables on the attributes of CSLNs quality. In this study, the factors under investigation were compritol 888 ATO (C-888, X1), poloxamer 188 (PL188, X2), and chitosan (CS, X3), which served as independent variables. The parameters measured as dependent variables included particle size (Y1), zeta potential (Y2), and encapsulation efficiency EE (Y3). The relationship among these variables was determined by Analysis of Variance (ANOVA) and response surface plots. The results revealed that PL188 played a significant role in reducing the particle size of CSLNS (ranging from 207 to 261 nm with 1 % PL188 to 167-229 nm with 3 % PL188). Conversely, an increase in the concentration of CS led to a rise in the particle size. The magnitude of positive zeta potential values was dependent on the increased concentration of CS. Moreover, the higher amounts of C-888 and PL188 improved the EE% of the CSLNs from 42 % to 86 %. Furthermore, a concentration-dependent antioxidant effect of the optimized AP-CSLNs was observed. The antioxidant activity of the optimized AP-CSLNs at 100 μg/mL was 75 % compared to 62 % and 60 % for AP-SLNs and AP solution, respectively. A similar pattern of improvement was also observed with antimicrobial, and anticancer activities of the optimized AP-CSLNs. These findings demonstrated the potential of AP-CSLNs as a carrier system, enhancing the biological activities of AP, opening new possibilities in herbal medicines.

Keywords: Aloe perryi; Anticancer; Antimicrobial; Antioxidant; CS; Evaluation; Nanoparticles; Optimization; SLNs.

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Conflict of interest statement

The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Tahany Saleh Aldayel reports financial support was provided by 10.13039/501100004242Princess Nourah bint Abdulrahman University. The authors declare no conflict of interest.

Figures

Fig. 1
Fig. 1
Estimation of response surface plot of the effect of independent factors on AP-CSLNs; particle size (A), zeta potential (B), and EE % (C).
Fig. 2
Fig. 2
Desirability of the optimized AP loaded CSLNs and the target responses.
Fig. 3
Fig. 3
In vitro release pattern of AP-loaded SLNs and CSLNs in PBS (pH 7.4) with 0.2 % TW80 (A) and TEM images of the optimized AP-CSLNs formulation (B).
Fig. 4
Fig. 4
Antioxidant efficacy of different concentrations of AP-CSLNs, AP-SLNs, and AP solution.
Fig. 5
Fig. 5
Antibacterial activity of AP extract, AP-SLNPs, AP-CSLNs, and positive control against (A) S. aureus, (B) P. aeruginosa, and (C) E. coli.
Fig. 6
Fig. 6
Cytotoxicity analysis of different cell lines(A) LoVo, (B) MCF-7, and A549 (C) treated with AP-SLN and AP-CSLNs at various concentrations for 48 h.
See this image and copyright information in PMC

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References

    1. Aldayel T.S., Alshammari G.M., Omar U.M., Grace M.H., Lila M.A., Yahya M.A. Hypoglycaemic, insulin releasing, and hepatoprotective effect of the aqueous extract of aloe perryi baker resin (Socotran aloe) in streptozotocin-induced diabetic rats. J. Taibah Univ. Sci. 2020;14:1671–1685.
    1. Aldayel T.S., Grace M.H., Lila M.A., Yahya M.A., Omar U.M., Alshammary G. LC-MS characterization of bioactive metabolites from two Yemeni aloe spp. with antioxidant and antidiabetic properties. Arab. J. Chem. 2020;13:5040–5049.
    1. Kim H.S., Kwak G.Y., Kim M., et al. Aloe perryi extracts promote proliferation and migration of human keratinocytes in vitro and skin wound healing in vivo. Int. J. Mol. Sci. 2018;19:1594. - PubMed
    1. Al-Oqail M.M., El-Shaibany A., Al-Jassas E., Al-Sheddi E.S., Al-Massarani S.M., Farshori N.N. In Vitro anti-proliferative activities of aloe perryi flowers extract on human liver, colon, breast, lung, prostate and epithelial cancer cell lines. Pak. J. Pharm. Sci. 2016;29:723–729. - PubMed
    1. Aldayel T.S., Badran M.M., Alomrani A.H., AlFaris N.A., Altamimi J.Z., Alqahtani A.S., Nasr F.A., Ghaffar S.A., Orfali R.S. Chitosan-coated solid lipid nanoparticles as an efficient avenue for boosted biological activities of aloe perryi: antioxidant, antibacterial, and anticancer potential. Molecules. 2023;28:3569. - PMC - PubMed