Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Feb;416(4):983-992.
doi: 10.1007/s00216-023-05088-6. Epub 2023 Dec 21.

Sensitive fluorescence ELISA for the detection of zearalenone based on self-assembly DNA nanocomposites and copper nanoclusters

Xudong Jing  1 , Sha Yu  1 , Ganggang Zhang  2 , Yanyan Tang  1 , Jiaqi Yin  1 , Juan Peng  1 , Weihua Lai  3
Affiliations

Sensitive fluorescence ELISA for the detection of zearalenone based on self-assembly DNA nanocomposites and copper nanoclusters

Xudong Jing et al. Anal Bioanal Chem. 2024 Feb.

Abstract

Zearalenone (ZEN), produced by Fusarium species, is a potential risk to human health. Traditional enzyme-linked immunosorbent assay (ELISA) is restricted due to low sensitivity for the detection of ZEN. Herein, enzyme nanocomposites (ALP-SA-Bio-ssDNA, ASBD) were prepared with the self-assembly strategy based on streptavidin-labeled alkaline phosphatase (SA-ALP) and dual-biotinylated ssDNA (B2-ssDNA). The enzyme nanocomposites improved the loading amount of ALP and catalyzed more ascorbic acid 2-phosphate to generate ascorbic acid (AA). Subsequently, Cu2+ could be reduced to copper nanoclusters (CuNCs) having strong fluorescence signal by AA with poly T. Benefiting from the high enzyme load of nanocomposites and the strong signal of CuNCs, the fluorescence ELISA was successfully established for the detection of ZEN. The proposed method exhibited lower limit of detection (0.26 ng mL-1) than traditional ELISA (1.55 ng mL-1). The recovery rates ranged from 92.00% to 108.38% (coefficient of variation < 9.50%) for the detection of zearalenone in corn and wheat samples. In addition, the proposed method exhibited no cross reaction with four other mycotoxins. This proposed method could be used in trace detection for food safety.

Keywords: Copper nanoclusters; Fluorescence enzyme-linked immunosorbent assay; Self-assembly strategy; Zearalenone.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Anfossi L, Baggiani C, Giovannoli C, D’Arco G, Giraudi G. Lateral-flow immunoassays for mycotoxins and phycotoxins: a review. Anal Bioanal Chem. 2013;405(2–3):467–80. https://doi.org/10.1007/s00216-012-6033-4 . - DOI - PubMed
    1. Smith MC, Madec S, Coton E, Hymery N. Natural co-occurrence of mycotoxins in foods and feeds and their in vitro combined toxicological effects. Toxins. 2016;8(4):94. https://doi.org/10.3390/toxins8040094 . - DOI - PubMed - PMC
    1. Xu SL, Zhang GG, Fang BL, Xiong QR, Duan HW, Lai WH. Lateral flow immunoassay based on polydopamine-coated gold nanoparticles for the sensitive detection of zearalenone in maize. Acs Appl Mater Inter. 2019;11(34):31283–90. https://doi.org/10.1021/acsami.9b08789 . - DOI
    1. Kong WJ, Li JY, Qiu F, Wei JH, Xiao XH, Zheng YG, Yang MH. Development of a sensitive and reliable high performance liquid chromatography method with fluorescence detection for high-throughput analysis of multi-class mycotoxins in Coix seed. Anal Chim Acta. 2013;799:68–76. https://doi.org/10.1016/j.aca.2013.08.042 . - DOI - PubMed
    1. Cho HD, Suh JH, Feng S, Eom T, Kim J, Hyun SM, Kim J, Wang Y, Han SB. Comprehensive analysis of multi-class mycotoxins in twenty different species of functional and medicinal herbs using liquid chromatography-tandem mass spectrometry. Food Control. 2019;96:517–26. https://doi.org/10.1016/j.foodcont.2018.10.007 . - DOI

LinkOut - more resources