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. 2023 Dec 4;15(4):684-695.
doi: 10.3390/hematolrep15040069.

Platelet Microvesicles, Inflammation, and Coagulation Markers: A Pilot Study

Antonio Gidaro  1 , Alessandro Palmerio Delitala  2 , Roberto Manetti  2 , Sonia Caccia  1 , Mark J Soloski  3 , Giorgio Lambertenghi Deliliers  4 , Dante Castro  2 , Mattia Donadoni  1 , Arianna Bartoli  1 , Giuseppe Sanna  2 , Luigi Bergamaschini  1 , Roberto Castelli  2
Affiliations

Platelet Microvesicles, Inflammation, and Coagulation Markers: A Pilot Study

Antonio Gidaro et al. Hematol Rep. .

Abstract

Background: Platelet "Microvesicles" (MVs) are studied for their role in blood coagulation and inflammation. The study aimed to establish if MVs are related to age, plasma levels of inflammation, coagulation, and fibrinolysis markers in healthy individuals.

Methods: We prospectively enrolled volunteers aged over 18 years. MVs, plasma levels of C-reactive protein (CRP), Interleukin 6 (IL-6), Interleukin 10 (IL-10), Interleukin 17 (IL-17), and transforming growth factor β (TGF-β), fibrinogen, plasminogen activator inhibitor-1 (PAI-1), von Willebrand factor (VWF), homocysteine, factor VII (FVII), thrombin activatable fibrinolysis inhibitor (TAFI), and Protein S were tested.

Results: A total of 246 individuals (median age 65 years ("IQR"54-72)) were evaluated. Both univariate analysis and logistic regression models showed that MVs positively correlate with age, CRP, IL-6, IL-10, IL-17, TGF-β, fibrinogen, PAI-1, VWF, FVII, and homocysteine, while inversely correlating with TAFI and Protein S. The ROC curve analysis performed to identify a cut off for MV values (700 kMP) showed a good accuracy with over-range cytokines fibrinolysis factor and coagulation markers.

Conclusions: To the best of our knowledge, this study is the first to correlate MVs with an entire panel of cardiovascular risk factors in healthy individuals. A future possible role of MVs in screening exams is suggested.

Keywords: C-reactive protein (CRP); Interleukin 10 (IL-10); Interleukin 17 (IL-17); Interleukin 6 (IL-6); Protein S; factor VII (FVII); fibrinogen; homocysteine; microparticles; microvesicles; plasminogen activator inhibitor-1 (PAI-1); thrombin activatable fibrinolysis inhibitor (TAFI); transforming growth factor β (TGF-β); von Willebrand factor (VWF).

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
(A–G): Results of AUC of ROC curve analysis for coagulation parameters.
Figure 1
Figure 1
(A–G): Results of AUC of ROC curve analysis for coagulation parameters.
Figure 2
Figure 2
(A–D): Results of AUC of ROC curve analysis for cytokines parameters.
See this image and copyright information in PMC

References

    1. Wolf P. The nature and significance of platelet products in human plasma. Br. J. Haematol. 1967;13:269–288. doi: 10.1111/j.1365-2141.1967.tb08741.x. - DOI - PubMed
    1. Sims P.J., Faioni E., Wiedmer T., Shattil S. Complement proteins C5b-9 cause release of membrane vesicles from the platelet surface that are enriched in the membrane receptor for coagulation factor Va and express prothrombinase activity. J. Biol. Chem. 1988;263:18205–18212. doi: 10.1016/S0021-9258(19)81346-7. - DOI - PubMed
    1. Ratajczak M.Z., Ratajczak D., Pedziwiatr D. Extracellular Microvesicles (ExMVs) in Cell to Cell Communication: A Role of Telocytes. Adv. Exp. Med. Biol. 2016;913:41–49. doi: 10.1007/978-981-10-1061-3_3. - DOI - PubMed
    1. Ratajczak M.Z., Ratajczak J. Extracellular Microvesicles as Game Changers in Better Understanding the Complexity of Cellular Interactions-From Bench to Clinical Applications. Am. J. Med. Sci. 2017;354:449–452. doi: 10.1016/j.amjms.2017.06.001. - DOI - PMC - PubMed
    1. Giesen P.L.A., Rauch U., Bohrmann B., Kling D., Roqué M., Fallon J.T., Badimon J.J., Himber J., Riederer M.A., Nemerson Y. Blood-borne tissue factor: Another view of thrombosis. Proc. Natl. Acad. Sci. USA. 1999;96:2311–2315. doi: 10.1073/pnas.96.5.2311. - DOI - PMC - PubMed