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. 2024:2749:39-54.
doi: 10.1007/978-1-0716-3609-1_4.

Salivary Organotypic Tissue Culture: An Ex-vivo 3D Model for Studying Radiation-Induced Injury of Human Salivary Glands

Akshaya Upadhyay  1 , Migmar Tsamchoe  2   3 , Simon D Tran  4
Affiliations

Salivary Organotypic Tissue Culture: An Ex-vivo 3D Model for Studying Radiation-Induced Injury of Human Salivary Glands

Akshaya Upadhyay et al. Methods Mol Biol. 2024.

Abstract

An organotypic tissue culture model can maintain the cellular and molecular interactions, as well as the extracellular components of a tissue ex vivo. Thus, this 3D model biologically mimics in vivo conditions better than commonly used 2D culture in vitro models. Here, we provide a detailed workflow for generating live 3D organotypic tissue slices from patient-derived freshly resected salivary glandular tissues. We also cover the processing of these tissues for various downstream applications like live-dead viability/cytotoxicity assay, FFPE sectioning and immunostaining, and RNA and protein extraction with a focus on the salivary gland radiation injury model. These procedures can be applied extensively to various solid organs and used for disease modeling for cancer research, radiation biology, and regenerative medicine.

Keywords: 3D tissue culture; Disease modeling; Ex vivo models; Organotypic slice culture; Radiation injury; Salivary gland regeneration.

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References

    1. Su X, Liu Y, Bakkar M, ElKashty O, El-Hakim M, Seuntjens J, Tran SD (2020) Labial stem cell extract mitigates injury to irradiated salivary glands. J Dent Res 99(3):293–301. https://doi.org/10.1177/0022034519898138 - DOI - PubMed
    1. Parker JJ, Lizarraga M, Waziri A, Foshay KM (2017) A human glioblastoma organotypic slice culture model for study of tumor cell migration and patient-specific effects of anti-invasive drugs. J Vis Exp 125. https://doi.org/10.3791/53557
    1. Kondru N, Manne S, Kokemuller R, Greenlee J, Greenlee MHW, Nichols T, Kong Q, Anantharam V, Kanthasamy A, Halbur P, Kanthasamy AG (2020) An ex vivo brain slice culture model of chronic wasting disease: implications for disease pathogenesis and therapeutic development. Sci Rep 10(1):7640. https://doi.org/10.1038/s41598-020-64456-9 - DOI - PubMed - PMC
    1. Falsig J, Sonati T, Herrmann US, Saban D, Li B, Arroyo K, Ballmer B, Liberski PP, Aguzzi A (2012) Prion pathogenesis is faithfully reproduced in cerebellar organotypic slice cultures. PLoS Pathog 8(11):e1002985. https://doi.org/10.1371/journal.ppat.1002985 - DOI - PubMed - PMC
    1. Misra S, Moro CF, Del Chiaro M, Pouso S, Sebestyén A, Löhr M, Björnstedt M, Verbeke CS (2019) Ex vivo organotypic culture system of precision-cut slices of human pancreatic ductal adenocarcinoma. Science 9(1):2133. https://doi.org/10.1038/s41598-019-38603-w - DOI

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