Comparative Study
doi: 10.1016/0003-9861(87)90004-x.
Biochemical characterization and development of DNA polymerases alpha and delta in the neonatal rat heart
- PMID: 3813535
- DOI: 10.1016/0003-9861(87)90004-x
Item in Clipboard
Comparative Study
Biochemical characterization and development of DNA polymerases alpha and delta in the neonatal rat heart
Arch Biochem Biophys.
1987 Jan.
Abstract
The ontogeny of DNA polymerase activity in the neonatal rat heart was studied. The DNA polymerase activities in rat heart extracts were identified as DNA polymerase alpha and DNA polymerase delta activities by their purification and characterization, by the use of a specific inhibitor (BuAdATP), and by a specific monoclonal antibody against DNA polymerase alpha. Using these inhibitors, it was shown that the two activities declined in parallel during the maturation and terminal differentiation of the heart.
Similar articles
-
Do DNA polymerases delta and alpha act coordinately as leading and lagging strand replicases?FEBS Lett. 1988 Feb 29;229(1):6-10. doi: 10.1016/0014-5793(88)80786-5. FEBS Lett. 1988. PMID: 3345838
-
Induction of DNA polymerase activities in the regenerating rat liver.Biochemistry. 1991 Jul 30;30(30):7534-41. doi: 10.1021/bi00244a024. Biochemistry. 1991. PMID: 1677271
-
Calf thymus DNA polymerase delta: purification, biochemical and functional properties of the enzyme after its separation from DNA polymerase alpha, a DNA dependent ATPase and proliferating cell nuclear antigen.Nucleic Acids Res. 1988 Jul 25;16(14A):6279-95. doi: 10.1093/nar/16.14.6279. Nucleic Acids Res. 1988. PMID: 2899882 Free PMC article.
-
Isolation and characterization of DNA polymerases from eukaryotic cells.Methods Cell Biol. 1978;19:27-42. doi: 10.1016/s0091-679x(08)60007-2. Methods Cell Biol. 1978. PMID: 357914 Review. No abstract available.
-
Mammalian DNA polymerases alpha and delta: current status in DNA replication.Biochemistry. 1988 Jun 28;27(13):4591-5. doi: 10.1021/bi00413a001. Biochemistry. 1988. PMID: 3048386 Review. No abstract available.
Cited by
-
Down-regulation of replication factor C-40 (RFC40) causes chromosomal missegregation in neonatal and hypertrophic adult rat cardiac myocytes.PLoS One. 2012;7(6):e39009. doi: 10.1371/journal.pone.0039009. Epub 2012 Jun 14. PLoS One. 2012. PMID: 22720015 Free PMC article.
-
Adult rat cardiomyocyte proliferation assay.In Vitro Cell Dev Biol Anim. 1997 Jun;33(6):428-31. doi: 10.1007/s11626-997-0060-z. In Vitro Cell Dev Biol Anim. 1997. PMID: 9201510 No abstract available.
-
Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase delta.Nucleic Acids Res. 1992 Feb 25;20(4):735-45. doi: 10.1093/nar/20.4.735. Nucleic Acids Res. 1992. PMID: 1542570 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
