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. 2023 Dec 16;12(12):2127.
doi: 10.3390/antiox12122127.

Chemical Composition and Biological Properties of New Romanian Lavandula Species

Ionuț Georgică Marchidan  1 , Alina Ortan  2 , Simona Marcu Spinu  2 , Sorin Marius Avramescu  3 , Ionela Avram  4 , Radu Claudiu Fierascu  5 , Narcisa Babeanu  1
Affiliations

Chemical Composition and Biological Properties of New Romanian Lavandula Species

Ionuț Georgică Marchidan et al. Antioxidants (Basel). .

Abstract

The aims of the present study were to evaluate for the first time the chemical composition and antioxidant, antibacterial, antifungal and antiproliferative potentials of the Romanian George 90 lavender species, as well as parental species, L. angustifolia and L. latifolia. The L. angustifolia, L. latifolia and George 90 essential oils were analyzed by GC-MS/MS and the L. angustifolia, L. latifolia and George 90 hydroalcoholic extracts were analyzed by HPLC-DAD. The antioxidant, antibacterial, antifungal and antiproliferative assays revealed that all the investigated species showed significant activities. The results highlighted the chemical composition and the promising biological potentials of the L. angustifolia, L. latifolia and George 90 lavender species, validating their ethnomedicinal value, which offers potential applications as natural drugs.

Keywords: Romanian George 90; chemical composition; lavender species; promising biological potential.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Images from the sampling site of the studied lavender species. L. angustifolia—Horodnic de Sus nursery, L. latifolia and George 90—Buftea nursery.
Figure 2
Figure 2
TIC chromatograms of (a) LA-MAE and (b) LA-HD essential oils.
Figure 2
Figure 2
TIC chromatograms of (a) LA-MAE and (b) LA-HD essential oils.
Figure 3
Figure 3
TIC chromatograms of (a) LL-MAE and (b) LL-HD essential oils.
Figure 4
Figure 4
TIC chromatograms of (a) G90-MAE and (b) G90-HD essential oils.
Figure 5
Figure 5
HPLC-DAD chromatograms of (a) LA, (b) LL and (c) G90 70% EtOH extracts, plant material particle size G2, plant material/solvent ratio 1:40, detected at a λ of 255 nm, 280 nm, 325 nm and 355 nm.
Figure 6
Figure 6
DPPH antioxidant potential expressed as antioxidant activity equivalents of (a) LA, LL and G90 essential oils and (b) LA, (c) LL and (d) G90 hydroalcoholic extracts. LA-MAE—L. angustifolia MAE essential oil, LA-HD—L. angustifolia HD essential oil, LL-MAE—L. latifolia MAE essential oil, LL-HD—L. latifolia HD essential oil, G90-MAE—George 90 MAE essential oil and G90-HD—George 90 HD essential oil. 1—EtOH 50%, plant material/solvent ratio at 1:20 (m/v), G1 coarse-sized plant material; 2—EtOH 50%, plant material/solvent ratio at 1:20 (m/v), G2 fine-sized plant material; 3—EtOH 50%, plant material/solvent ratio at 1:40 (m/v), G1 coarse-sized plant material; 4—EtOH 50%, plant material/solvent ratio at 1:40 (m/v), G2 fine-sized plant material; 5—EtOH 70%, plant material/solvent ratio at 1:20 (m/v), G1 coarse-sized plant material; 6—EtOH 70%, plant material/solvent ratio at 1:20 (m/v), G2 fine-sized plant material; 7—EtOH 70%, plant material/solvent ratio at 1:40 (m/v), G1 coarse-sized plant material; 8—EtOH 70%, plant material/solvent ratio at 1:40 (m/v), G2 fine-sized plant material; 9—MeOH 50%, plant material/solvent ratio at 1:20 (m/v), G1 coarse-sized plant material; 10—MeOH 50%, plant material/solvent ratio at 1:20 (m/v), G2 fine-sized plant material; 11—MeOH 50%, plant material/solvent ratio at 1:40 (m/v), G1 coarse-sized plant material; 12—MeOH 50%, plant material/solvent ratio at 1:40 (m/v), G2 fine-sized plant material. Values are means ± SD, n = 3 per treatment group. Means in a bar without a common superscript letter differ (p < 0.05), as analyzed by one-way ANOVA and the TUKEY test.
Figure 6
Figure 6
DPPH antioxidant potential expressed as antioxidant activity equivalents of (a) LA, LL and G90 essential oils and (b) LA, (c) LL and (d) G90 hydroalcoholic extracts. LA-MAE—L. angustifolia MAE essential oil, LA-HD—L. angustifolia HD essential oil, LL-MAE—L. latifolia MAE essential oil, LL-HD—L. latifolia HD essential oil, G90-MAE—George 90 MAE essential oil and G90-HD—George 90 HD essential oil. 1—EtOH 50%, plant material/solvent ratio at 1:20 (m/v), G1 coarse-sized plant material; 2—EtOH 50%, plant material/solvent ratio at 1:20 (m/v), G2 fine-sized plant material; 3—EtOH 50%, plant material/solvent ratio at 1:40 (m/v), G1 coarse-sized plant material; 4—EtOH 50%, plant material/solvent ratio at 1:40 (m/v), G2 fine-sized plant material; 5—EtOH 70%, plant material/solvent ratio at 1:20 (m/v), G1 coarse-sized plant material; 6—EtOH 70%, plant material/solvent ratio at 1:20 (m/v), G2 fine-sized plant material; 7—EtOH 70%, plant material/solvent ratio at 1:40 (m/v), G1 coarse-sized plant material; 8—EtOH 70%, plant material/solvent ratio at 1:40 (m/v), G2 fine-sized plant material; 9—MeOH 50%, plant material/solvent ratio at 1:20 (m/v), G1 coarse-sized plant material; 10—MeOH 50%, plant material/solvent ratio at 1:20 (m/v), G2 fine-sized plant material; 11—MeOH 50%, plant material/solvent ratio at 1:40 (m/v), G1 coarse-sized plant material; 12—MeOH 50%, plant material/solvent ratio at 1:40 (m/v), G2 fine-sized plant material. Values are means ± SD, n = 3 per treatment group. Means in a bar without a common superscript letter differ (p < 0.05), as analyzed by one-way ANOVA and the TUKEY test.
Figure 7
Figure 7
ABTS antioxidant potential expressed as the antioxidant activity equivalents of (a) LA, LL and G90 essential oils and (b) LA, (c) LL and (d) G90 hydroalcoholic extracts. LA-MAE—L. angustifolia MAE essential oil, LA-HD—L. angustifolia HD essential oil, LL-MAE—L. latifolia MAE essential oil, LL-HD—L. latifolia HD essential oil, G90-MAE—George 90 MAE essential oil and G90-HD—George 90 HD essential oil. 1—EtOH 50%, plant material/solvent ratio at 1:20 (m/v), G1 coarse-sized plant material; 2—EtOH 50%, plant material/solvent ratio at 1:20 (m/v), G2 fine-sized plant material; 3—EtOH 50%, plant material/solvent ratio at 1:40 (m/v), G1 coarse-sized plant material; 4—EtOH 50%, plant material/solvent ratio at 1:40 (m/v), G2 fine-sized plant material; 5—EtOH 70%, plant material/solvent ratio at 1:20 (m/v), G1 coarse-sized plant material; 6—EtOH 70%, plant material/solvent ratio at 1:20 (m/v), G2 fine-sized plant material; 7—EtOH 70%, plant material/solvent ratio at 1:40 (m/v), G1 coarse-sized plant material; 8—EtOH 70%, plant material/solvent ratio at 1:40 (m/v), G2 fine-sized plant material; 9—MeOH 50%, plant material/solvent ratio at 1:20 (m/v), G1 coarse-sized plant material; 10—MeOH 50%, plant material/solvent ratio at 1:20 (m/v), G2 fine-sized plant material; 11—MeOH 50%, plant material/solvent ratio at 1:40 (m/v), G1 coarse-sized plant material; 12—MeOH 50%, plant material/solvent ratio at 1:40 (m/v), G2 fine-sized plant material. Values are presented as means ± SD, n = 3 per treatment group. Data without a common superscript letter differ (p < 0.05), as analyzed by one-way ANOVA and the TUKEY test.
Figure 8
Figure 8
FRAP antioxidant potential expressed as antioxidant activity equivalents of (a) LA, LL and G90 essential oils and (b) LA, (c) LL and (d) G90 hydroalcoholic extracts. LA-MAE—L. angustifolia MAE essential oil, LA-HD—L. angustifolia HD essential oil, LL-MAE—L. latifolia MAE essential oil, LL-HD—L. latifolia HD essential oil, G90-MAE—George 90 MAE essential oil and G90-HD—George 90 HD essential oil. 1—EtOH 50%, plant material/solvent ratio at 1:20 (m/v), G1 coarse-sized plant material; 2—EtOH 50%, plant material/solvent ratio at 1:20 (m/v), G2 fine-sized plant material; 3—EtOH 50%, plant material/solvent ratio at 1:40 (m/v), G1 coarse-sized plant material; 4—EtOH 50%, plant material/solvent ratio at 1:40 (m/v), G2 fine-sized plant material; 5—EtOH 70%, plant material/solvent ratio at 1:20 (m/v), G1 coarse-sized plant material; 6—EtOH 70%, plant material/solvent ratio at 1:20 (m/v), G2 fine-sized plant material; 7—EtOH 70%, plant material/solvent ratio at 1:40 (m/v), G1 coarse-sized plant material; 8—EtOH 70%, plant material/solvent ratio at 1:40 (m/v), G2 fine-sized plant material; 9—MeOH 50%, plant material/solvent ratio at 1:20 (m/v), G1 coarse-sized plant material; 10—MeOH 50%, plant material/solvent ratio at 1:20 (m/v), G2 fine-sized plant material; 11—MeOH 50%, plant material/solvent ratio at 1:40 (m/v), G1 coarse-sized plant material; 12—MeOH 50%, plant material/solvent ratio at 1:40 (m/v), G2 fine-sized plant material. Values are means ± SD, n = 3 per treatment group. Means in a bar without a common superscript letter differ (p < 0.05), as analyzed by one-way ANOVA and the TUKEY test.
Figure 8
Figure 8
FRAP antioxidant potential expressed as antioxidant activity equivalents of (a) LA, LL and G90 essential oils and (b) LA, (c) LL and (d) G90 hydroalcoholic extracts. LA-MAE—L. angustifolia MAE essential oil, LA-HD—L. angustifolia HD essential oil, LL-MAE—L. latifolia MAE essential oil, LL-HD—L. latifolia HD essential oil, G90-MAE—George 90 MAE essential oil and G90-HD—George 90 HD essential oil. 1—EtOH 50%, plant material/solvent ratio at 1:20 (m/v), G1 coarse-sized plant material; 2—EtOH 50%, plant material/solvent ratio at 1:20 (m/v), G2 fine-sized plant material; 3—EtOH 50%, plant material/solvent ratio at 1:40 (m/v), G1 coarse-sized plant material; 4—EtOH 50%, plant material/solvent ratio at 1:40 (m/v), G2 fine-sized plant material; 5—EtOH 70%, plant material/solvent ratio at 1:20 (m/v), G1 coarse-sized plant material; 6—EtOH 70%, plant material/solvent ratio at 1:20 (m/v), G2 fine-sized plant material; 7—EtOH 70%, plant material/solvent ratio at 1:40 (m/v), G1 coarse-sized plant material; 8—EtOH 70%, plant material/solvent ratio at 1:40 (m/v), G2 fine-sized plant material; 9—MeOH 50%, plant material/solvent ratio at 1:20 (m/v), G1 coarse-sized plant material; 10—MeOH 50%, plant material/solvent ratio at 1:20 (m/v), G2 fine-sized plant material; 11—MeOH 50%, plant material/solvent ratio at 1:40 (m/v), G1 coarse-sized plant material; 12—MeOH 50%, plant material/solvent ratio at 1:40 (m/v), G2 fine-sized plant material. Values are means ± SD, n = 3 per treatment group. Means in a bar without a common superscript letter differ (p < 0.05), as analyzed by one-way ANOVA and the TUKEY test.
Figure 9
Figure 9
Antiproliferative potential of LA, LL and G90 essential oils against the HeLa tumoral cell line (a,b). Data are the mean ± SD of four replicates per condition. LA-MAE—L. angustifolia MAE essential oil, LA-HD—L. angustifolia HD essential oil, LL-MAE—L. latifolia MAE essential oil, LL-HD—L. latifolia HD essential oil, G90-MAE—George 90 MAE essential oil and G90-HD—George 90 HD essential oil. Values are means ± SD, n = 3 per treatment group. Means in a bar without a common superscript letter differ (p < 0.05), as analyzed by one-way ANOVA and the TUKEY test.
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