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. 2023 Dec 10;13(12):1770.
doi: 10.3390/biom13121770.

Kinetics of Phosphate Ions and Phytase Activity Production for Lactic Acid-Producing Bacteria Utilizing Milling and Whitening Stages Rice Bran as Biopolymer Substrates

Rojarej Nunta  1   2 , Julaluk Khemacheewakul  1   3 , Charin Techapun  1   3 , Sumeth Sommanee  1   3 , Juan Feng  1   3 , Su Lwin Htike  1   3 , Chatchadaporn Mahakuntha  1   3 , Kritsadaporn Porninta  1   3 , Yuthana Phimolsiripol  1   3 , Kittisak Jantanasakulwong  1   3 , Churairat Moukamnerd  3 , Masanori Watanabe  4 , Anbarasu Kumar  1   3   5 , Noppol Leksawasdi  1   3
Affiliations

Kinetics of Phosphate Ions and Phytase Activity Production for Lactic Acid-Producing Bacteria Utilizing Milling and Whitening Stages Rice Bran as Biopolymer Substrates

Rojarej Nunta et al. Biomolecules. .

Abstract

A study evaluated nine kinetic data and four kinetic parameters related to growth, production of various phytase activities (PEact), and released phosphate ion concentration ([Pi]) from five lactic acid bacteria (LAB) strains cultivated in three types of media: phytate (IP6), milling stage rice bran (MsRB), and whitening stage rice bran (WsRB). Score ranking techniques were used, combining these kinetic data and parameters to select the most suitable LAB strain for each medium across three cultivation time periods (24, 48, and 72 h). In the IP6 medium, Lacticaseibacillus casei TISTR 1500 exhibited statistically significant highest (p ≤ 0.05) normalized summation scores using a 2:1 weighting between kinetic and parameter data sets. This strain also had the statistically highest levels (p ≤ 0.05) of produced phosphate ion concentration ([Pi]) (0.55 g/L) at 72 h and produced extracellular specific phytase activity (ExSp-PEact) (0.278 U/mgprotein) at 48 h. For the MsRB and WsRB media, Lactiplantibacillus plantarum TISTR 877 performed exceptionally well after 72 h of cultivation. It produced ([Pi], ExSp-PEact) pairs of (0.53 g/L, 0.0790 U/mgprotein) in MsRB and (0.85 g/L, 0.0593 U/mgprotein) in WsRB, respectively. Overall, these findings indicate the most promising LAB strains for each medium and cultivation time based on their ability to produce phosphate ions and extracellular specific phytase activity. The selection process utilized a combination of kinetic data and parameter analysis.

Keywords: lactic acid bacteria; phytic acid; rice bran; solid waste; sustainability.

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Conflict of interest statement

The authors declare no competing interest.

Figures

Figure 1
Figure 1
Kinetic data and parameter profiles as well as normalized weighting scores of five LAB strains (TISTR ■ 1500; ● 877; ▲ 890; ♦ 055; × 1498) during 72 h cultivation time in IP6 medium with respect to (a) [IP6]; (b) μ; (c) produced [Pi]; (d) qp,Pi; (e) produced ExSp-PEact; and (f) normalized scores. Each SE was included as an error bar for each data point. All [IP6] existed in [IP6]sol form. The tabulated average and error results values for each LAB strain cultivation with a statistically significant comparison between time points of (a) are in Supplementary Section B Table S1.1; (b) in Table S1.6; (c) in Table S1.1; (d) in Table S1.6; (e) in Table S1.3; and (f) in Table S1.6. The statistically significant comparisons in (b,d) were made across three cultivation time intervals for each LAB strain with similar font coloring. The statistically significant comparison in (f) was made within each type of summation; DSc; PSc; DSc + PSc; 2DSc + PSc. The numbers with the same alphabet (A–C; a–d) indicate no statistically significant difference (p > 0.05).
Figure 2
Figure 2
Kinetic data and parameter profiles as well as normalized weighting scores of five LAB strains (TISTR ■ 1500; ● 877; ▲ 890; ▲ 890; ♦ 055; × 1498) during 72 h cultivation time in MsRB medium with respect to (a) [IP6]overall; (b) μ; (c) produced [Pi]; (d) qp,Pi; (e) produced ExSp-PEact; and (f) normalized scores. Each SE was included as an error bar for each data point. [IP6]overall existed in both [IP6]sol and [IP6]in-sol forms. The tabulated average and error results values for each LAB strain cultivation with a statistically significant comparison between time points of (a) are in Supplementary Section B Table S2.1; (b) in Table S2.6; (c) in Table S2.1; (d) in Table S2.6; (e) in Table S2.3; and (f) in Table S2.6. The statistically significant comparisons in (b,d) were made across three cultivation time intervals for each LAB strain with similar font coloring. The statistically significant comparison in (f) was made within each type of summation; DSc; PSc; DSc + PSc; 2DSc + PSc. The numbers with the same alphabet (A–C; a–d) indicate no statistically significant difference (p > 0.05).
Figure 3
Figure 3
Kinetic data and parameter profiles as well as normalized weighting scores of five LAB strains (TISTR ■ 1500; ● 877; ▲ 890; ♦ 055; × 1498) during 72 h cultivation time in WsRB medium with respect to (a) [IP6]overall; (b) μ; (c) produced [Pi]; (d) qp,Pi; (e) produced ExSp-PEact; and (f) normalised scores. Each SE was included as an error bar for each data point. [IP6]overall existed in both [IP6]sol and [IP6]in-sol forms. The tabulated average and error results values for each LAB strain cultivation with statistically significant comparison between time points of (a) are in Supplementary Section B Table S3.1; (b) in Table S3.6; (c) in Table S3.1; (d) in Table S3.6; (e) in Table S3.3; and (f) in Table S3.6. The statistically significant comparisons in (b,d) were made across three cultivation time intervals for each LAB strain with similar font coloring. The statistically significant comparison in (f) was made within each type of summation; DSc; PSc; DSc + PSc; 2DSc + PSc. The numbers with the same alphabet (A–C; a–d) indicate no statistically significant difference (p > 0.05).
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