Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Dec 1;240(2):593-6.
doi: 10.1042/bj2400593.

The characterization of an acidic calmodulin-binding protein in brain cytoskeleton and membrane fractions

P Strocchi, J M Gilbert

The characterization of an acidic calmodulin-binding protein in brain cytoskeleton and membrane fractions

P Strocchi et al. Biochem J. .

Abstract

One of the most abundant acidic proteins in rat brain has an Mr of 68,000 and a pI of 5.6 (68K 5.6 protein) when analysed by two-dimensional gel electrophoresis. The 68K 5.6 protein was found in large relative amounts in brain cytoskeleton preparations and in membrane and supernatant fractions. High-salt washing and proteolytic digestion did not remove this protein from the membrane elements. The 68K 5.6 protein was also found in the microtubule-associated protein fraction of purified microtubules and was present in large relative amounts in preparations of intermediate-filament proteins. The 68K 5.6 protein binds to calmodulin in the presence of Ca2+ ions, and we found it to be an abundant acidic calmodulin-binding protein in brain tissue.

PubMed Disclaimer

References

    1. J Neurochem. 1981 Nov;37(5):1295-307 - PubMed
    1. FEBS Lett. 1984 Jul 9;172(2):315-20 - PubMed
    1. J Biol Chem. 1982 Aug 25;257(16):9663-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1982 Jul;79(13):4002-5 - PubMed
    1. J Cell Biol. 1982 Jul;94(1):129-42 - PubMed

Publication types