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. 1986 Dec 30;141(3):1242-8.
doi: 10.1016/s0006-291x(86)80178-4.

Expression of Agrobacterium tumefaciens T-DNA gene 7 in Xenopus laevis oocytes

J C McPherson, R Kingsbury

Expression of Agrobacterium tumefaciens T-DNA gene 7 in Xenopus laevis oocytes

J C McPherson et al. Biochem Biophys Res Commun. .

Abstract

The expression of Agrobacterium tumefaciens T-DNA gene 7 was investigated in Xenopus laevis oocytes. Cloned DNA injected into oocytes consisted of T-DNA sequences derived from octopine type Ti plasmid B6-806 and T-DNA attached to plant DNA sequences at the left junction in crown gall tumors. Transcription initiation sites observed in oocytes were similar to those for transcript 7 in crown gall tumors. Quantitative differences in transcription occurred depending on the flanking sequences of the injected clones indicating that sequences upstream of the TATA box of T-DNA gene 7 affect the quantitative expression of this gene in Xenopus oocytes.

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