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Review
. 2023 Dec 8:10:1289676.
doi: 10.3389/fvets.2023.1289676. eCollection 2023.

Current detection methods of African swine fever virus

Zhiqiang Hu #  1   2   3   4 , Xiaogang Tian #  1   2   3 , Ranran Lai #  1   2   3 , Xinglong Wang  5 , Xiaowen Li  1   2   3   4   5   6
Affiliations
Review

Current detection methods of African swine fever virus

Zhiqiang Hu et al. Front Vet Sci. .

Abstract

African swine fever (ASF), caused by the African swine fever virus (ASFV), is a highly contagious and notifiable animal disease in domestic pigs and wild boars, as designated by the World Organization for Animal Health (WOAH). The effective diagnosis of ASF holds great importance in promptly controlling its spread due to its increasing prevalence and the continuous emergence of variant strains. This paper offers a comprehensive review of the most common and up-to-date methods established for various genes/proteins associated with ASFV. The discussed methods primarily focus on the detection of viral genomes or particles, as well as the detection of ASFV associated antibodies. It is anticipated that this paper will serve as a reference for choosing appropriate diagnostic methods in diverse application scenarios, while also provide direction for the development of innovative technologies in the future.

Keywords: African swine fever virus; antibodies; detection method; laboratory testing; on-site testing; viral genomes.

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Conflict of interest statement

ZH, XT, RL, XL were employed by Xiajin New Hope Liuhe Agriculture and Animal Husbandry Co., Ltd., Shandong New Hope Liuhe Co., Ltd., Shandong New Hope Liuhe Agriculture and Animal Husbandry Technology Co., Ltd., (NHLH Academy of Swine Research), and also XL was employed by New Hope Liuhe Co., Ltd. The remaining author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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