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. 1986:(11):30-3.

[Physicochemical characteristics of sorbitol dehydrogenase from the cytoplasm of the liver cells of susliks]

[Article in Russian]
V E Sudovtsov, L I Grechkina
  • PMID: 3814659

[Physicochemical characteristics of sorbitol dehydrogenase from the cytoplasm of the liver cells of susliks]

[Article in Russian]
V E Sudovtsov et al. Nauchnye Doki Vyss Shkoly Biol Nauki. 1986.

Abstract

Some physico-chemical properties of sorbitol dehydrogenase from squirrel liver cell cytoplasm have been investigated. Non-linear dependence of enzyme activity upon media pH is shown. Activity manifests only in the presence of NAD that can't be replaced by NADP. The enzyme exhibits stereospecificity: it dehydrates polyol of any length, the second and the fourth carbon atoms have common L-configuration concerning the first carbon atom. A diffusion zone with Rf = 0,09 is revealed on the electrophoregram. Three thin zones of activity about pH 7,2 are exposed by isoelectrofocusing method.

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