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. 2024 Jan;30(1):168-171.
doi: 10.3201/eid3001.230756.

Avian Influenza A(H5N1) Neuraminidase Inhibition Antibodies in Healthy Adults after Exposure to Influenza A(H1N1)pdm09

Pavithra Daulagala, Samuel M S Cheng, Alex Chin, Leo L H Luk, Kathy Leung, Joseph T Wu, Leo L M Poon, Malik Peiris, Hui-Ling Yen

Avian Influenza A(H5N1) Neuraminidase Inhibition Antibodies in Healthy Adults after Exposure to Influenza A(H1N1)pdm09

Pavithra Daulagala et al. Emerg Infect Dis. 2024 Jan.

Abstract

We detected high titers of cross-reactive neuraminidase inhibition antibodies to influenza A(H5N1) virus clade 2.3.4.4b in 96.8% (61/63) of serum samples from healthy adults in Hong Kong in 2020. In contrast, antibodies at low titers were detected in 42% (21/50) of serum samples collected in 2009. Influenza A(H1N1)pdm09 and A(H5N1) titers were correlated.

Keywords: clade 2.3.4.4.b; cross-reactive antibody response; influenza; influenza A(H1N1)pdm09; influenza A(H5N1); neuraminidase inhibition antibody; pandemic risk assessment; respiratory infections; viruses.

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Figures

Figure
Figure
Age-stratified HAI and NAI antibody responses to influenza A(H1N1)pdm09 (California/09) and A(H5N1) (Spoonbill/HK/22) viruses in serum samples collected from healthy adults in 2020 and 2009, Hong Kong, China. A, B) Results for serum samples of 63 healthy adults collected in 2020. C, D) Results for serum samples of 50 healthy adults collected in 2009. The assay detection limit was 1:10, and samples with antibody below the detection limit were assigned an arbitrary antibody titer of 5, which is used to calculate geometric mean titer. The HAI and NAI titers across different age groups were compared using Kruskal-Wallis test and Dunn’s multiple comparison test. HAI, hemagglutination inhibition; NAI, neuraminidase inhibition.
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References

    1. Lee DH, Bertran K, Kwon JH, Swayne DE. Evolution, global spread, and pathogenicity of highly pathogenic avian influenza H5Nx clade 2.3.4.4. J Vet Sci. 2017;18(S1):269–80. 10.4142/jvs.2017.18.S1.269 - DOI - PMC - PubMed
    1. Pohlmann A, King J, Fusaro A, Zecchin B, Banyard AC, Brown IH, et al. Has epizootic become enzootic? Evidence for a fundamental change in the infection dynamics of highly pathogenic avian influenza in Europe, 2021. MBio. 2022;13:e0060922. 10.1128/mbio.00609-22 - DOI - PMC - PubMed
    1. Adlhoch C, Fusaro A, Gonzales JL, Kuiken T, Mirinaviciute G, Niqueux É, et al.; European Food Safety Authority, European Centre for Disease Prevention and Control, European Union Reference Laboratory for Avian Influenza. Avian influenza overview March - April 2023. EFSA J. 2023;21:e08039. - PubMed
    1. World Health Organization. Antigenic and genetic characteristics of zoonotic influenza A viruses and development of candidate vaccine viruses for pandemic preparedness. 2022. [cited 2023 May 18]. https://cdn.who.int/media/docs/default-source/influenza/who-influenza-re...
    1. Krammer F, Weir JP, Engelhardt O, Katz JM, Cox RJ. Meeting report and review: Immunological assays and correlates of protection for next-generation influenza vaccines. Influenza Other Respir Viruses. 2020;14:237–43. 10.1111/irv.12706 - DOI - PMC - PubMed