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. 2024;17(4):662-666.
doi: 10.1016/j.jcmgh.2023.12.006. Epub 2023 Dec 24.

Altered B-Cell Expansion and Maturation in Draining Mesenteric Lymph Nodes of Inflamed Gut in Crohn's Disease

Sonja Kappel-Latif  1 , Prasanti Kotagiri  2 , Lukas Schlager  1 , Gabor Schuld  1 , Natalie Walterskirchen  1 , Vanessa Schimek  1 , Gavin Sewell  3 , Carina Binder  4 , Johanna Jobst  1 , Supriya Murthy  5 , Barbara Messner  6 , Stefanie Dabsch  7 , Arthur Kaser  3 , Paul A Lyons  2 , Michael Bergmann  1 , Anton Stift  1 , Rudolf Oehler  1 , Lukas W Unger  8
Affiliations

Altered B-Cell Expansion and Maturation in Draining Mesenteric Lymph Nodes of Inflamed Gut in Crohn's Disease

Sonja Kappel-Latif et al. Cell Mol Gastroenterol Hepatol. 2024.
No abstract available

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Figures

Figure 1
Figure 1
B cells expand within affected draining MLNs. (A–I) Characterization within paired draining MLNs of affected and healthy small intestinal segments. (A) Schematic depiction of sample collection. (B–E) Eighteen patients were included for basic immunologic phenotyping for (B) CD45+ cells, (C) CD45+CD14+ cells, (D) CD45+CD3+ cells, and (E) CD45+CD19+ cells. (F–I) A more detailed B-cell subset analysis of 9 patients showing (F) IgD+CD27+ marginal zone B cells, (G) IgD-CD27- double-negative B cells of CD45+CD19+ B cells, (H) CD38+ cells, and (I) CD38- cells of CD45+CD19+CD27+IgD- B cells. (J and K) Differences in GC (J) abundance and (K) size per high-power field (HPF). (L–Q) Immunohistochemistry (IHC) staining: representative image of a draining MLN of (L) healthy and (M) affected small intestine stained for CD19. Purple arrow and yellow arrow indicate GCs. MLN of a (N) healthy small intestine and (O) affected segment stained for Ki67. MLN of a (P) healthy small intestine and (Q) affected segment stained for Bcl6. (B–K) A paired Student t test was used for analysis. ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001.
Figure 2
Figure 2
B-cell–receptor sequencing indicates pathologic B-cell maturation. (A) Schematic depiction of the BCR sequencing strategy performed in 24 patients. (B) BCR isotype use in MLNs. (C) Rate of SHM in CDRs or framework regions (FWRs). (D) Rate of SHM is depicted as the ratio of affected/healthy MLNs. (E) Diversity analysis on BCR sequences of the variable regions. Richness refers to the abundance of unique clones in a repertoire, Simpson’s index assesses the probability of 2 randomly sampled reads belonging to the same clone, Shannon’s index is a measure of evenness. (F) Depiction of individual results and (E) as log of affected/healthy. ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001. freq., frequency; IGHA, Immunoglobulin Heavy Constant Alpha; IGHD, Immunoglobulin Heavy Constant Delta; IGHE, Immunoglobulin Heavy Constant Epsilon; IGHG, Immunoglobulin Heavy Constant Gamma; IGHM, Immunoglobulin Heavy Constant Mu.
See this image and copyright information in PMC

References

    1. Scheid J.F., et al. J Exp Med. 2023;220 - PMC - PubMed
    1. Uzzan M., et al. Nat Med. 2022;28:766–779. - PMC - PubMed
    1. Castro-Dopico T., et al. Immunity. 2019;50:1099–1114 e10. - PMC - PubMed
    1. Roda G., et al. Nat Rev Dis Primers. 2020;6:22. - PubMed
    1. Li Y., et al. Inflamm Bowel Dis. 2015;21:2613–2618. - PubMed

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