The Contribution of DNA Ligase 4 Polymorphisms to Colorectal Cancer

doi:10.21873/invivo.13419

. 2024 Jan-Feb;38(1):127-133.

doi: 10.21873/invivo.13419.

The Contribution of DNA Ligase 4 Polymorphisms to Colorectal Cancer

Yang Deng^{1

2

3}, Tao-Wei Ke^{4

5}, Te-Cheng Yueh^{5

6

7}, Yu-Ting Chin^{5

6}, Yun-Chi Wang^{5

6}, Yi-Chih Hung^{5

6}, Mei-Chin Mong⁸, Ya-Chen Yang⁸, Wen-Tzu Wu⁸, Wen-Shin Chang^{3

5

6}, Jian Gu^#⁹, DA-Tian Bau^#^{10

6

11}, Chia-Wen Tsai^#^{12

5

6}

Affiliations

¹ Department of General Surgery, Wuxi Branch of Shanghai Ruijin Hospital, Wuxi, P.R. China.
² Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China.
³ Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX, U.S.A.
⁴ Department of General Surgery, China Medical University Hospital, Taichung, Taiwan, R.O.C.
⁵ Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.
⁶ Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, R.O.C.
⁷ National Defense Medical Center, Taipei, Taiwan, R.O.C.
⁸ Department of Food Nutrition and Health Biotechnology, Asia University, Taichung, Taiwan, R.O.C.
⁹ Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX, U.S.A.; jiangu@mdanderson.org.
¹⁰ Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.; artbau2@gmail.com.
¹¹ Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan, R.O.C.
¹² Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX, U.S.A.; wenwen816@gmail.com.

^# Contributed equally.

PMID: 38148049
PMCID: PMC10756467
DOI: 10.21873/invivo.13419

The Contribution of DNA Ligase 4 Polymorphisms to Colorectal Cancer

Yang Deng et al. In Vivo. 2024 Jan-Feb.

. 2024 Jan-Feb;38(1):127-133.

doi: 10.21873/invivo.13419.

Authors

Affiliations

¹ Department of General Surgery, Wuxi Branch of Shanghai Ruijin Hospital, Wuxi, P.R. China.
² Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China.
³ Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX, U.S.A.
⁴ Department of General Surgery, China Medical University Hospital, Taichung, Taiwan, R.O.C.
⁵ Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.
⁶ Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, R.O.C.
⁷ National Defense Medical Center, Taipei, Taiwan, R.O.C.
⁸ Department of Food Nutrition and Health Biotechnology, Asia University, Taichung, Taiwan, R.O.C.
⁹ Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX, U.S.A.; jiangu@mdanderson.org.
¹⁰ Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.; artbau2@gmail.com.
¹¹ Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan, R.O.C.
¹² Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX, U.S.A.; wenwen816@gmail.com.

^# Contributed equally.

PMID: 38148049
PMCID: PMC10756467
DOI: 10.21873/invivo.13419

Abstract

Background/aim: While numerous biomarkers associated with genetic susceptibility to colorectal cancer (CRC) have been identified and validated through epidemiological studies, the specific influence of DNA ligase 4 (Lig4) genotypes remains unexplored. This study aimed to elucidate the hitherto unexamined relationship between Lig4 genotypes and CRC risk.

Materials and methods: The genotypes of Lig4 rs1805388 were determined applying the polymerase chain reaction-restriction fragment length polymorphism methodology. The potential association between these genotypes and CRC risk was assessed in a Taiwanese population comprising 362 CRC cases and an equal number of age- and sex-matched controls.

Results: In the genotypic analysis, the distribution of CC, CT, and TT genotypes for Lig4 rs1805388 among CRC cases was 54.7%, 38.1%, and 7.2%, respectively. This distribution was not significantly different from the controls, which exhibited genotypic frequencies of 57.2%, 36.7%, and 6.1%, respectively (p for trend=0.7314). Analysis of allelic distribution indicated that individuals carrying the T allele of Lig4 rs1805388 displayed a slightly elevated CRC risk compared to those carrying the C allele (odds ratio=1.10, 95% confidence interval=0.87-1.39, p=0.4685).

Conclusion: The variant genotypes of Lig4 rs1805388 may not serve as predictive markers for CRC risk in the Taiwanese population.

Keywords: Colorectal cancer; DNA ligase 4; genotypes; non-homologous end-joining; single nucleotide polymorphism.

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Conflict of interest statement

The Authors declare no conflicts of interest regarding this study.

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References

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1. Bau DT, Mau YC, Ding SL, Wu PE, Shen CY. DNA double-strand break repair capacity and risk of breast cancer. Carcinogenesis. 2007;28(8):1726–1730. doi: 10.1093/carcin/bgm109. - DOI - PubMed
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[1] Morgan E, Arnold M, Gini A, Lorenzoni V, Cabasag CJ, Laversanne M, Vignat J, Ferlay J, Murphy N, Bray F. Global burden of colorectal cancer in 2020 and 2040: incidence and mortality estimates from GLOBOCAN. Gut. 2023;72(2):338–344. doi: 10.1136/gutjnl-2022-327736. - DOI - PubMed

[2] Morgan E, Arnold M, Gini A, Lorenzoni V, Cabasag CJ, Laversanne M, Vignat J, Ferlay J, Murphy N, Bray F. Global burden of colorectal cancer in 2020 and 2040: incidence and mortality estimates from GLOBOCAN. Gut. 2023;72(2):338–344. doi: 10.1136/gutjnl-2022-327736. - DOI - PubMed

[3] Roelands J, van der Ploeg M, Ijsselsteijn ME, Dang H, Boonstra JJ, Hardwick JCH, Hawinkels LJAC, Morreau H, de Miranda NFCC. Transcriptomic and immunophenotypic profiling reveals molecular and immunological hallmarks of colorectal cancer tumourigenesis. Gut. 2023;72(7):1326–1339. doi: 10.1136/gutjnl-2022-327608. - DOI - PMC - PubMed

[4] Roelands J, van der Ploeg M, Ijsselsteijn ME, Dang H, Boonstra JJ, Hardwick JCH, Hawinkels LJAC, Morreau H, de Miranda NFCC. Transcriptomic and immunophenotypic profiling reveals molecular and immunological hallmarks of colorectal cancer tumourigenesis. Gut. 2023;72(7):1326–1339. doi: 10.1136/gutjnl-2022-327608. - DOI - PMC - PubMed

[5] Tsai CW, Shih LC, Chang WS, Hsu CL, He JL, Hsia TC, Wang YC, Gu J, Bau DT. Non-homologous end-joining pathway genotypes significantly associated with nasopharyngeal carcinoma susceptibility. Biomedicines. 2023;11(6):1648. doi: 10.3390/biomedicines11061648. - DOI - PMC - PubMed

[6] Tsai CW, Shih LC, Chang WS, Hsu CL, He JL, Hsia TC, Wang YC, Gu J, Bau DT. Non-homologous end-joining pathway genotypes significantly associated with nasopharyngeal carcinoma susceptibility. Biomedicines. 2023;11(6):1648. doi: 10.3390/biomedicines11061648. - DOI - PMC - PubMed

[7] Bau DT, Mau YC, Ding SL, Wu PE, Shen CY. DNA double-strand break repair capacity and risk of breast cancer. Carcinogenesis. 2007;28(8):1726–1730. doi: 10.1093/carcin/bgm109. - DOI - PubMed

[8] Bau DT, Mau YC, Ding SL, Wu PE, Shen CY. DNA double-strand break repair capacity and risk of breast cancer. Carcinogenesis. 2007;28(8):1726–1730. doi: 10.1093/carcin/bgm109. - DOI - PubMed

[9] Bau DT, Fu YP, Chen ST, Cheng TC, Yu JC, Wu PE, Shen CY. Breast cancer risk and the DNA double-strand break end-joining capacity of nonhomologous end-joining genes are affected by BRCA1. Cancer Res. 2004;64(14):5013–5019. doi: 10.1158/0008-5472.CAN-04-0403. - DOI - PubMed

[10] Bau DT, Fu YP, Chen ST, Cheng TC, Yu JC, Wu PE, Shen CY. Breast cancer risk and the DNA double-strand break end-joining capacity of nonhomologous end-joining genes are affected by BRCA1. Cancer Res. 2004;64(14):5013–5019. doi: 10.1158/0008-5472.CAN-04-0403. - DOI - PubMed

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The Contribution of DNA Ligase 4 Polymorphisms to Colorectal Cancer

Affiliations

The Contribution of DNA Ligase 4 Polymorphisms to Colorectal Cancer

Authors

Affiliations

Abstract

Conflict of interest statement

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Abstract

Conflict of interest statement

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