The Contribution of DNA Ligase 4 Polymorphisms to Colorectal Cancer

Yang Deng^{1

2

3}, Tao-Wei Ke^{4

5}, Te-Cheng Yueh^{5

6

7}, Yu-Ting Chin^{5

6}, Yun-Chi Wang^{5

6}, Yi-Chih Hung^{5

6}, Mei-Chin Mong⁸, Ya-Chen Yang⁸, Wen-Tzu Wu⁸, Wen-Shin Chang^{3

5

6}, Jian Gu^#⁹, DA-Tian Bau^#^{10

6

11}, Chia-Wen Tsai^#^{12

5

6}

Affiliations

¹ Department of General Surgery, Wuxi Branch of Shanghai Ruijin Hospital, Wuxi, P.R. China.
² Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China.
³ Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX, U.S.A.
⁴ Department of General Surgery, China Medical University Hospital, Taichung, Taiwan, R.O.C.
⁵ Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.
⁶ Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, R.O.C.
⁷ National Defense Medical Center, Taipei, Taiwan, R.O.C.
⁸ Department of Food Nutrition and Health Biotechnology, Asia University, Taichung, Taiwan, R.O.C.
⁹ Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX, U.S.A.; jiangu@mdanderson.org.
¹⁰ Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.; artbau2@gmail.com.
¹¹ Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan, R.O.C.
¹² Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX, U.S.A.; wenwen816@gmail.com.

^# Contributed equally.

PMID: 38148049
PMCID: PMC10756467
DOI: 10.21873/invivo.13419

The Contribution of DNA Ligase 4 Polymorphisms to Colorectal Cancer

Yang Deng et al. In Vivo. 2024 Jan-Feb.

. 2024 Jan-Feb;38(1):127-133.

doi: 10.21873/invivo.13419.

Authors

Affiliations

¹ Department of General Surgery, Wuxi Branch of Shanghai Ruijin Hospital, Wuxi, P.R. China.
² Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China.
³ Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX, U.S.A.
⁴ Department of General Surgery, China Medical University Hospital, Taichung, Taiwan, R.O.C.
⁵ Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.
⁶ Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, R.O.C.
⁷ National Defense Medical Center, Taipei, Taiwan, R.O.C.
⁸ Department of Food Nutrition and Health Biotechnology, Asia University, Taichung, Taiwan, R.O.C.
⁹ Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX, U.S.A.; jiangu@mdanderson.org.
¹⁰ Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.; artbau2@gmail.com.
¹¹ Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan, R.O.C.
¹² Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX, U.S.A.; wenwen816@gmail.com.

^# Contributed equally.

PMID: 38148049
PMCID: PMC10756467
DOI: 10.21873/invivo.13419

Abstract

Background/aim: While numerous biomarkers associated with genetic susceptibility to colorectal cancer (CRC) have been identified and validated through epidemiological studies, the specific influence of DNA ligase 4 (Lig4) genotypes remains unexplored. This study aimed to elucidate the hitherto unexamined relationship between Lig4 genotypes and CRC risk.

Materials and methods: The genotypes of Lig4 rs1805388 were determined applying the polymerase chain reaction-restriction fragment length polymorphism methodology. The potential association between these genotypes and CRC risk was assessed in a Taiwanese population comprising 362 CRC cases and an equal number of age- and sex-matched controls.

Results: In the genotypic analysis, the distribution of CC, CT, and TT genotypes for Lig4 rs1805388 among CRC cases was 54.7%, 38.1%, and 7.2%, respectively. This distribution was not significantly different from the controls, which exhibited genotypic frequencies of 57.2%, 36.7%, and 6.1%, respectively (p for trend=0.7314). Analysis of allelic distribution indicated that individuals carrying the T allele of Lig4 rs1805388 displayed a slightly elevated CRC risk compared to those carrying the C allele (odds ratio=1.10, 95% confidence interval=0.87-1.39, p=0.4685).

Conclusion: The variant genotypes of Lig4 rs1805388 may not serve as predictive markers for CRC risk in the Taiwanese population.

Keywords: Colorectal cancer; DNA ligase 4; genotypes; non-homologous end-joining; single nucleotide polymorphism.

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Conflict of interest statement

The Authors declare no conflicts of interest regarding this study.

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The Contribution of DNA Ligase 4 Polymorphisms to Colorectal Cancer

Affiliations

The Contribution of DNA Ligase 4 Polymorphisms to Colorectal Cancer

Authors

Affiliations

Abstract

Conflict of interest statement

References

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Medical

Molecular Biology Databases

Research Materials