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. 2023 Nov-Dec;175(6):e14087.
doi: 10.1111/ppl.14087.

Broad-spectrum antagonistic potential of Bacillus spp. volatiles against Rhizoctonia solani and Xanthomonas oryzae pv. oryzae

Qurban Ali  1 , Abdur Rashid Khan  1 , Sheng Tao  1 , Faheem Uddin Rajer  2 , Muhammad Ayaz  1 , Manzoor Ali Abro  2 , Qin Gu  1 , Huijun Wu  1 , Vladislav Kuptsov  3 , Emilia Kolomiets  3 , Xuewen Gao  1
Affiliations

Broad-spectrum antagonistic potential of Bacillus spp. volatiles against Rhizoctonia solani and Xanthomonas oryzae pv. oryzae

Qurban Ali et al. Physiol Plant. 2023 Nov-Dec.

Abstract

Rhizoctonia solani and Xanthomonas oryzae pv. oryzae (Xoo) are the two major diseases affecting the quality and quantity of rice production. In the current study, volatile organic compounds (VOCs) of Bacillus spp. were used as green biocontrol agents for plant diseases. In in vitro experiments, Bacillus spp. FZB42, NMTD17, and LLTC93-VOCs displayed strong antimicrobial volatile activity with inhibition rates of 76, 66, and 78% for R. solani and 78, 81, and 76% for Xoo, respectively, compared to control. The synthetic VOCs, namely Pentadecane (PDC), Benzaldehyde (BDH), 1,2-Benz isothiazol-3(2H)-one (1,2-BIT), and mixture (MIX) of VOCs showed high volatile activity with inhibition rates of 86, 86, 89, and 92% against R. solani and 81, 81, 82, and 86%, respectively, against Xoo as compared to control. In addition, the scanning and transmission electron microscopes (SEM and TEM) analyses were performed to examine the effect of Bacillus and synthetic VOC treatments on R. solani and Xoo morphology. The analysis revealed the deformed and irregularized morphology of R. solani mycelia and Xoo cells after VOC treatments. The microscopic analysis showed that the rapid inhibition was due to severe oxidative productions inside the R. solani mycelia and Xoo cells. By using molecular docking, it was determined that the synthetic VOCs entered the active binding site of trehalase and NADH dehydrogenase proteins, causing R. solani and Xoo cells to die prematurely and an accumulation of ROS. In the greenhouse experiment, FZB42, NMTD17, and LLTC93-VOCs significantly reduced the lesions of R. solani 8, 7, and 6 cm, and Xoo 7, 6, and 6 cm, respectively, then control. The synthetic VOCs demonstrated that the PDC, BDH, 1,2-BIT, and MIX-VOCs significantly reduced R. solani lesions on leaves 6, 6, 6, and 5 cm and Xoo 6, 5, 5, and 4 cm, respectively, as compared to control. Furthermore, plant defence-related genes and antioxidant enzymes were upregulated in rice plants. These findings provide novel mechanisms by which Bacillus antimicrobial VOCs control plant diseases.

Keywords: Antimicrobial volatile activity; Bacillus spp.; Molecular docking; biocontrol; plant defence genes.

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References

REFERENCES

    1. Adhikari TB, Joseph CM, Yang G, Phillips DA, Nelson LM (2001) Evaluation of bacteria isolated from rice for plant growth promotion and biological control of seedling disease of rice. Can J Microbiol 47: 916-924.
    1. Aktar MW, Sengupta D, Chowdhury A (2009) Impact of pesticides use in agriculture: their benefits and hazards. Interdiscip Toxicol 2: 1.
    1. Ali Q, Ayaz M, Mu G, Hussain A, Yuanyuan Q, Yu C, Xu Y, Manghwar H, Gu Q, Wu H, Gao X (2022a) Revealing plant growth-promoting mechanisms of Bacillus strains in elevating rice growth and its interaction with salt stress. 1-17.
    1. Ali Q, Ayaz M, Yu C, Wang Y, Gu Q, Wu H, Gao X (2022b) Cadmium tolerant microbial strains possess different mechanisms for cadmium biosorption and immobilization in rice seedlings. Chemosphere 303: 135206.
    1. Ali Q, Yu C, Wang Y, Sheng T, Zhao X, Wu X, Jing L, Gu Q, Wu H, Gao X (2023b) High killing rate of nematode and promotion of rice growth by Synthetic Volatiles from Bacillus Strains due to enhanced oxidative stress response. Physiol Plant e13868.

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