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. 2023 Dec;125(Pt A):111033.
doi: 10.1016/j.intimp.2023.111033. Epub 2023 Oct 27.

Rutin attenuates inflammation by downregulating AGE-RAGE signaling pathway in psoriasis: Network pharmacology analysis and experimental evidence

Mingxia Wang  1 , Xiaoxuan Ma  2 , Chunjie Gao  1 , Yue Luo  1 , Xiaoya Fei  1 , Qi Zheng  1 , Xin Ma  3 , Le Kuai  4 , Bin Li  3 , Ruiping Wang  5 , Jiankun Song  6
Affiliations

Rutin attenuates inflammation by downregulating AGE-RAGE signaling pathway in psoriasis: Network pharmacology analysis and experimental evidence

Mingxia Wang et al. Int Immunopharmacol. 2023 Dec.

Abstract

Background: Jueyin granules (JYG) is effective against psoriasis, but its utility components are not clear. Rutin is the main monomer of JYG, its therapeutic effect and mechanism on psoriasis need to be further clarified.

Purpose: To explore the potential mechanisms of rutin on psoriasis through network pharmacology and experiments.

Methods: In vitro, cell viability was determined using the CCK8 assay, and inflammatory factors were identified using RT-qPCR. The hub genes and kernel pathways of action were identified by modular pharmacology analysis. In vivo, a BALB/c mice model of psoriasis was induced by Imiquimod (IMQ). The therapeutic effect and action pathway were detected through Western Blotting, RT-qPCR, histopathologic and immunohistochemical analysis.

Results: Rutin inhibited cell proliferation and expression of TNF-α and IL-6 in HaCaT cells. The hub genes include APP, INS, and TNF, while the kernel pathways contain the AGE-RAGE signaling pathway. In IMQ-induced psoriasis-like mice, rutin ameliorated skin lesions and inhibited cell proliferation. Rutin could attenuate inflammation by downregulating the AGE-RAGE signaling pathway.

Conclusion: This study suggests that rutin can reduce IMQ-induced psoriasis like skin inflammation in mice, and regulation of AGE-RAGE signaling pathway may be one of its potential anti-inflammatory mechanisms. Rutin has a promising therapeutic use for the treatment of psoriasis.

Keywords: AGE-RAGE signaling pathway; Network pharmacology; Psoriasis; Rutin.

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Conflict of interest statement

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.