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. 2023 Dec 1;2(4):042401.
doi: 10.1149/2754-2726/ad15a1. Epub 2023 Dec 27.

Perspective-Assessing Electrochemical, Aptamer-Based Sensors for Dynamic Monitoring of Cellular Signaling

Celeste R Rousseau  1 , Hope Kumakli  1 , Ryan J White  1   2
Affiliations

Perspective-Assessing Electrochemical, Aptamer-Based Sensors for Dynamic Monitoring of Cellular Signaling

Celeste R Rousseau et al. ECS Sens Plus. .

Abstract

Electrochemical, aptamer-based (E-AB) sensors provide a generalizable strategy to quantitatively detect a variety of targets including small molecules and proteins. The key signaling attributes of E-AB sensors (sensitivity, selectivity, specificity, and reagentless and dynamic sensing ability) make them well suited to monitor dynamic processes in complex environments. A key bioanalytical challenge that could benefit from the detection capabilities of E-AB sensors is that of cell signaling, which involves the release of molecular messengers into the extracellular space. Here, we provide a perspective on why E-AB sensors are suited for this measurement, sensor requirements, and pioneering examples of cellular signaling measurements.

Keywords: aptamer; cell signaling; electrochemical aptamer-based sensors; electrochemistry.

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Figures

Figure 1.
Figure 1.
A possible scheme for aptamer target binding. The average distance between the redox reporter and the electrode surface will be different in the bound compared to the unbound state, thus resulting in a measurable change in current.
See this image and copyright information in PMC

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