Clinical pilot study on microfluidic automation of IGH-VJ library preparation for next generation sequencing

Jacob F Hess^{1

2}, Michaela Kotrová³, Birgit Fricke³, Simona Songia⁴, Silvia Rigamonti⁴, Roberta Cavagna⁵, Manuela Tosi⁵, Nils Paust^{1

2}, Anton W Langerak⁶, Orietta Spinelli⁵, Giovanni Cazzaniga^{4

7}, Monika Brüggemann³, Tobias Hutzenlaub^{1

2}

Affiliations

¹ Hahn-Schickard, Freiburg, Germany.
² Laboratory for MEMS Applications, IMTEK - Department of Microsystems Engineering, University of Freiburg, Freiburg, Germany.
³ Unit for Hematological Diagnostics, II. Medical Department, University Medical Center Schleswig Holstein, Kiel, Germany.
⁴ Centro Tettamanti, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy.
⁵ Struttura Complessa Ematologia, ASST Papa Giovanni XXIII, Bergamo, Italy.
⁶ Department of Immunology, Laboratory Medical Immunology, Erasmus MC University Medical Center, Rotterdam, The Netherlands.
⁷ School of Medicine and Surgery, University of Milan-Bicocca, Monza, Italy.

Jacob F Hess et al. Clin Chem Lab Med. 2023.

. 2023 Dec 29;62(7):e164-e167.

doi: 10.1515/cclm-2023-1346. Print 2024 Jun 25.

¹ Hahn-Schickard, Freiburg, Germany.
² Laboratory for MEMS Applications, IMTEK - Department of Microsystems Engineering, University of Freiburg, Freiburg, Germany.
³ Unit for Hematological Diagnostics, II. Medical Department, University Medical Center Schleswig Holstein, Kiel, Germany.
⁴ Centro Tettamanti, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy.
⁵ Struttura Complessa Ematologia, ASST Papa Giovanni XXIII, Bergamo, Italy.
⁶ Department of Immunology, Laboratory Medical Immunology, Erasmus MC University Medical Center, Rotterdam, The Netherlands.
⁷ School of Medicine and Surgery, University of Milan-Bicocca, Monza, Italy.

No abstract available

Keywords: automation; high-throughput nucleotide sequencing; microfluidics; precursor cell lymphoblastic leukemia-lymphoma.

References

1. Yohe, S, Thyagarajan, B. Review of clinical next-generation sequencing. Arch Pathol Lab Med 2017;141:1544–57. https://doi.org/10.5858/arpa.2016-0501-ra . - DOI
1. Hess, JF, Kohl, TA, Kotrová, M, Rönsch, K, Paprotka, T, Mohr, V, et al.. Library preparation for next generation sequencing: a review of automation strategies. Biotechnol Adv 2020;41:107537. https://doi.org/10.1016/j.biotechadv.2020.107537 . - DOI
1. Besser, J, Carleton, HA, Gerner-Smidt, P, Lindsey, RL, Trees, E. Next-generation sequencing technologies and their application to the study and control of bacterial infections. Clin Microbiol Infect 2018;24:335–41. https://doi.org/10.1016/j.cmi.2017.10.013 . - DOI
1. Kim, H, Jebrail, MJ, Sinha, A, Bent, ZW, Solberg, OD, Williams, KP, et al.. A microfluidic DNA library preparation platform for next-generation sequencing. PLoS One 2013;8:e68988. https://doi.org/10.1371/journal.pone.0068988 . - DOI
1. Hess, JF, Hess, ME, Zengerle, R, Paust, N, Boerries, M, Hutzenlaub, T. Automated library preparation for whole genome sequencing by centrifugal microfluidics. Anal Chim Acta 2021;1182:338954. https://doi.org/10.1016/j.aca.2021.338954 . - DOI