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. 2024 Mar 6;14(3):jkad296.
doi: 10.1093/g3journal/jkad296.

Whole-genome sequence and annotation of Penstemon davidsonii

Kate L Ostevik  1   2 , Magdy Alabady  3 , Mengrui Zhang  4 , Mark D Rausher  2
Affiliations

Whole-genome sequence and annotation of Penstemon davidsonii

Kate L Ostevik et al. G3 (Bethesda). .

Abstract

Penstemon is the most speciose flowering plant genus endemic to North America. Penstemon species' diverse morphology and adaptation to various environments have made them a valuable model system for studying evolution. Here, we report the first full reference genome assembly and annotation for Penstemon davidsonii. Using PacBio long-read sequencing and Hi-C scaffolding technology, we constructed a de novo reference genome of 437,568,744 bases, with a contig N50 of 40 Mb and L50 of 5. The annotation includes 18,199 gene models, and both the genome and transcriptome assembly contain over 95% complete eudicot BUSCOs. This genome assembly will serve as a valuable reference for studying the evolutionary history and genetic diversity of the Penstemon genus.

Keywords: Penstemon davidsonii; Davidson's beardtongue; Hi-C; PacBio Hifi; genome annotation; genome assembly.

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Conflict of interest statement

Conflicts of interest The author(s) declare no conflicts of interest.

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