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. 2023 Dec 1;24(12):4071-4076.
doi: 10.31557/APJCP.2023.24.12.4071.

Methylation Status of CDH1 Gene in Gastric Biopsy Specimens and Its Association with H. Pylori Infection

Karima Ali Hamid  1 , Ryan Sidig Adam Abdalgalil  1 , Yousif Abdelhameed Mohammed  2 , Rania Mahgob  1 , Khalid Abdelsamea Mohamedahmed  3 , Bakri Yousif Mohamed Nour  4
Affiliations

Methylation Status of CDH1 Gene in Gastric Biopsy Specimens and Its Association with H. Pylori Infection

Karima Ali Hamid et al. Asian Pac J Cancer Prev. .

Abstract

Background: Helicobacter pylori (H. pylori) have been accepted as having an etiologic role in gastro-duodenal diseases as chronic gastritis, peptic ulcer, and gastric carcinoma. Methylation of CGI has been correlated with the tumorigenic process since it can inactivate tumor suppressor genes. CDH1 is a tumor suppressor gene that encodes the E-cadherin protein, which is preserving cell-cell connections. Early stages of gastric carcinogenesis may be affected by the promoter methylation-mediated inactivation of this gene.

Objective: This study aimed to investigate the methylation status of CDH1 using Methylation-Specific PCR (MSP) technique in clinical suspected patients with H. pylori infection who undergoing upper gastrointestinal endoscopy and correlated it with H. pylori detection by glmM PCR test.

Methods: Fifty gastric mucosal biopsies were selected from one hundred and five samples included in this study. The detection of H. pylori was performed with the PCR primers specific to glmM gene. Bisulfite modification was done and the methylation status of the CDH1 gene was detected using MSP reaction.

Results: H. pylori was detected in 36% (18/50) of study population using glmM gene PCR test, 89% (16/18) of H. pylori positive cases were CDH1 methylated positive (chi-square, p-value=0.002). CDH1 methylation can be present in cancerous and noncancerous gastric mucosa, where 60% (18/30) of CDH1 methylation positive gastric mucosa showed gastritis as an endoscopy finding and gastric cancer in 6% (2/30). There was a significant correlation between and CDH1 methylation positive results and age group (P-value = 0.02). There was no significant correlation between CDH1 methylation positive results and participants gender (p-value=0.431) and clinical symptoms (all P-value > 0.05).

Conclusion: This work suggested strong significance association between H. pylori infection and CDH1 methylation.

Keywords: CDH1 methylation; Gastric cancer; H. pylori.

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Conflict of interest statement

The authors declare that they have no conflict of interests.

Figures

Figure 1
Figure 1
Gel Electrophoresis Representative MSP PCR Products of CDH1Gene. DNA ladder = 100 bp, samples 1 was positive in both methylated (M) and unmethylated (U), sample 2, 3 and 4 were positive just only in methylated (M)
See this image and copyright information in PMC

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