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. 2024 Mar;108(3):116163.
doi: 10.1016/j.diagmicrobio.2023.116163. Epub 2023 Dec 17.

Non-specific signals in real-time RT-PCR for detecting respiratory viruses

Gannon C K Mak  1 , Derek L L Hung  2
Affiliations

Non-specific signals in real-time RT-PCR for detecting respiratory viruses

Gannon C K Mak et al. Diagn Microbiol Infect Dis. 2024 Mar.

Abstract

We describe our experiences in investigating the origin of non-specific signals during the development phase of a multiplex PCR assay for respiratory viruses. After ruling out various sources of error, eventually we discovered the non-specific signal was related to the particular lot of the PCR kit.

Keywords: Non-specific signals; PCR; Reagent lot.

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Conflict of interest statement

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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