[A Preliminary Study on the Plasma Metabolomic Profiles of Patients Suffering From Acute Diquat Poisoning]

doi:10.12182/20231160601

. 2023 Nov 20;54(6):1219-1226.

doi: 10.12182/20231160601.

[A Preliminary Study on the Plasma Metabolomic Profiles of Patients Suffering From Acute Diquat Poisoning]

[Article in Chinese]

Hui Hu^{1

2

3}, Yu DU^{1

2

3}

Affiliations

¹ / （ 610041） Department of Emergency and Critical Care Medicine,West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
² / （ 610041） Department of Occupational and Environmental Health, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
³ - （ 610041） Health Emergency Management Research Center, West China-PUMC C.C. Chen Institute of Health, Sichuan University, Chengdu 610041, China.

PMID: 38162068
PMCID: PMC10752779
DOI: 10.12182/20231160601

[A Preliminary Study on the Plasma Metabolomic Profiles of Patients Suffering From Acute Diquat Poisoning]

[Article in Chinese]

Hui Hu et al. Sichuan Da Xue Xue Bao Yi Xue Ban. 2023.

. 2023 Nov 20;54(6):1219-1226.

doi: 10.12182/20231160601.

Authors

Hui Hu^{1

2

3}, Yu DU^{1

2

3}

Affiliations

¹ / （ 610041） Department of Emergency and Critical Care Medicine,West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
² / （ 610041） Department of Occupational and Environmental Health, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
³ - （ 610041） Health Emergency Management Research Center, West China-PUMC C.C. Chen Institute of Health, Sichuan University, Chengdu 610041, China.

PMID: 38162068
PMCID: PMC10752779
DOI: 10.12182/20231160601

Abstract
in English, Chinese

Objective: To analyze the plasma metabolomic features of patients suffering from acute diquat (DQ) poisoning and to explore the molecular mechanism and potential biomarkers of DQ poisoning.

Methods: A total of 7 patients suffering from acute DQ poisoning were enrolled in the DQ poisoning group. The poisoning of these patients occurred within a 12-h window at the time of enrollment. Meanwhile, 7 healthy immediate family members of the patients were enrolled as the normal controls. Liquid chromatography-mass spectrometry (LC-MS) was used to perform non-targeted metabolomic profiling of the plasma samples and to screen and identify differential metabolites and metabolic pathways.

Results: A total of 104 metabolites were screened and identified (P<0.05 and the variable importance in the projection [VIP]>1). Compared with those of the control group, 61 metabolites, such as sorbitol and galactitol, were up-regulated, and 43 metabolites, such as myo-inositol and gamma-glutamylcysteine, were down-regulated in the DQ poisoning group. Pathway enrichment analysis revealed changes in 11 metabolic pathways, including those for galactose metabolism and linoleic acid metabolism (P<0.05).

Conclusion: Metabolomics analysis of plasma samples from DQ poisoning patients shows that DQ mainly interferes with the metabolism of energy, amino acids, and lipids, thus causing metabolic disorders. Some potential biomarkers closely associated with oxidative stress and organ damage of the liver, kidney, and nervous system have been identified.

目的: 分析急性敌草快（diquat, DQ）中毒患者血浆代谢组学特征，探讨中毒的分子机制和潜在生物标志物。

方法: 收集7例12 h内急性DQ中毒的患者作为DQ中毒组，7例同时期患者直系健康家属为正常对照组。采用液相色谱-质谱联用（liquid chromatography-mass spectrometry, LC-MS）的非靶向代谢组学技术对血浆样本进行检测，筛选和鉴定差异代谢物及代谢通路。

结果: 筛选和鉴定出104个代谢物（P<0.05和VIP>1），与对照组相比，DQ中毒患者血浆中山梨醇、半乳糖醇等61个代谢物上调，肌-肌醇、γ-谷氨酰半胱氨酸等43个代谢物下调。通路富集分析发现有半乳糖代谢、亚油酸代谢等11条代谢通路发生变化（P<0.05）。

结论: 通过对DQ中毒患者血浆样本的代谢组学分析，发现DQ主要干扰了能量、氨基酸、脂质代谢，造成代谢紊乱，并发现若干与氧化应激、肝、肾、神经系统等器官损伤密切相关的潜在生物标志物。

Keywords: Diquat; Metabolomics; Plasma; Poisoning.

PubMed Disclaimer

Conflict of interest statement

利益冲突所有作者均声明不存在利益冲突

Figures

**图 2**
The Principal component analysis (PCA) of QC samples QC样本PCA得分图

**图 3**
Score plots of PCA model from the control (blue circle) and DQ groups (red circle) PCA得分图

**图 4**
Score plots of the OPLS-DA model from the control (blue circle) and DQ groups (red circle) OPLS-DA得分图

**图 5**
Hierarchical clustering heatmap of the differential metabolites 差异代谢物的层次聚类热图

**图 6**
Bubble chart of the impact of metabolic pathways 代谢通路影响因子气泡图

See this image and copyright information in PMC

References

1. 岳琳娜, 向平, 宋粉云, 等生物检材中常见除草剂的分析方法及研究进展. 法医学杂志. 2021;37(2):248–255. doi: 10.12116/j.issn.1004-5619.2019.390902. - DOI
1. 余锋, 林时辉, 廖晓辉, 等代谢组学分析在重症医学领域的研究与应用进展. 重庆医科大学学报. 2018;43(7):877–882. doi: 10.13406/j.cnki.cyxb.001670. - DOI
1. HERTZOG A, SELVANATHAN A, DEVANAPALLI B, et al A narrative review of metabolomics in the era of "-omics": integration into clinical practice for inborn errors of metabolism. Transl Pediatr. 2022;11(10):1704–1716. doi: 10.21037/tp-22-105. - DOI - PMC - PubMed
1. XIAO Y, LIN X, ZHOU M, et al Metabolomics analysis of the potential toxicological mechanisms of diquat dibromide herbicide in adult zebrafish (Danio rerio) liver. Fish Physiol Biochem. 2022;48(4):1039–1055. doi: 10.1007/s10695-022-01101-4. - DOI - PubMed
1. ZHANG H, ZHANG J, LI J, et al Multi-Omics Analyses reveal the mechanisms of early stage kidney toxicity by diquat. Toxics. 2023;11(2):184. doi: 10.3390/toxics11020184. - DOI - PMC - PubMed

Publication types

Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions
Actions

LinkOut - more resources

Full Text Sources
- Europe PubMed Central
- PubMed Central

[1] 岳琳娜, 向平, 宋粉云, 等生物检材中常见除草剂的分析方法及研究进展. 法医学杂志. 2021;37(2):248–255. doi: 10.12116/j.issn.1004-5619.2019.390902. - DOI

[2] 岳琳娜, 向平, 宋粉云, 等生物检材中常见除草剂的分析方法及研究进展. 法医学杂志. 2021;37(2):248–255. doi: 10.12116/j.issn.1004-5619.2019.390902. - DOI

[3] 余锋, 林时辉, 廖晓辉, 等代谢组学分析在重症医学领域的研究与应用进展. 重庆医科大学学报. 2018;43(7):877–882. doi: 10.13406/j.cnki.cyxb.001670. - DOI

[4] 余锋, 林时辉, 廖晓辉, 等代谢组学分析在重症医学领域的研究与应用进展. 重庆医科大学学报. 2018;43(7):877–882. doi: 10.13406/j.cnki.cyxb.001670. - DOI

[5] HERTZOG A, SELVANATHAN A, DEVANAPALLI B, et al A narrative review of metabolomics in the era of "-omics": integration into clinical practice for inborn errors of metabolism. Transl Pediatr. 2022;11(10):1704–1716. doi: 10.21037/tp-22-105. - DOI - PMC - PubMed

[6] HERTZOG A, SELVANATHAN A, DEVANAPALLI B, et al A narrative review of metabolomics in the era of "-omics": integration into clinical practice for inborn errors of metabolism. Transl Pediatr. 2022;11(10):1704–1716. doi: 10.21037/tp-22-105. - DOI - PMC - PubMed

[7] XIAO Y, LIN X, ZHOU M, et al Metabolomics analysis of the potential toxicological mechanisms of diquat dibromide herbicide in adult zebrafish (Danio rerio) liver. Fish Physiol Biochem. 2022;48(4):1039–1055. doi: 10.1007/s10695-022-01101-4. - DOI - PubMed

[8] XIAO Y, LIN X, ZHOU M, et al Metabolomics analysis of the potential toxicological mechanisms of diquat dibromide herbicide in adult zebrafish (Danio rerio) liver. Fish Physiol Biochem. 2022;48(4):1039–1055. doi: 10.1007/s10695-022-01101-4. - DOI - PubMed

[9] ZHANG H, ZHANG J, LI J, et al Multi-Omics Analyses reveal the mechanisms of early stage kidney toxicity by diquat. Toxics. 2023;11(2):184. doi: 10.3390/toxics11020184. - DOI - PMC - PubMed

[10] ZHANG H, ZHANG J, LI J, et al Multi-Omics Analyses reveal the mechanisms of early stage kidney toxicity by diquat. Toxics. 2023;11(2):184. doi: 10.3390/toxics11020184. - DOI - PMC - PubMed

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

[A Preliminary Study on the Plasma Metabolomic Profiles of Patients Suffering From Acute Diquat Poisoning]

Affiliations

[A Preliminary Study on the Plasma Metabolomic Profiles of Patients Suffering From Acute Diquat Poisoning]

Authors

Affiliations

Abstract
in English, Chinese

Conflict of interest statement

Figures

Similar articles

References

Publication types

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Abstract in English, Chinese

Conflict of interest statement

Figures

Similar articles

References

Publication types

MeSH terms

Substances

Related information

LinkOut - more resources

Full Text Sources

Abstract
in English, Chinese