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Comment
. 2024 Jan 2;134(1):e176672.
doi: 10.1172/JCI176672.

In vivo gene editing of CAMKIID: out with the bad and in with the good

John E Smith 3rd, Henk Granzier
Comment

In vivo gene editing of CAMKIID: out with the bad and in with the good

John E Smith 3rd et al. J Clin Invest. .

Abstract

The ability to change an organism's DNA through gene editing is of great importance for the prevention and treatment of genetic and acquired diseases. Rapid progress has been made during the last decade due to the discovery and refinement of CRISPR/Cas9 as an accurate, fast, and reliable genome editing technique. In this issue of the JCI, Lebek et al. present the culmination from a line of work in the Olson laboratory focused on in vivo gene editing of CAMK2D. The paper presents a combined state-of-the-art gene therapy approach that demonstrates how gene therapy can yield cardioprotection in a mouse model and takes notable steps toward potential applicability in patients.

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Conflict of interest statement

Conflict of interest: The authors declare that no conflict of interest exists.

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References

    1. Hidalgo CG, et al. The multifunctional Ca(2+)/calmodulin-dependent protein kinase II delta (CaMKIIδ) phosphorylates cardiac titin’s spring elements. J Mol Cell Cardiol. 2013;54:90–97. doi: 10.1016/j.yjmcc.2012.11.012. - DOI - PMC - PubMed
    1. Mattiazzi A, et al. Chasing cardiac physiology and pathology down the CaMKII cascade. Am J Physiol Heart Circ Physiol. 2015;308(10):H1177–H1191. doi: 10.1152/ajpheart.00007.2015. - DOI - PMC - PubMed
    1. Wu Y, et al. Myocardial death and dysfunction after ischemia-reperfusion injury require CaMKIIδ oxidation. Sci Rep. 2019;9(1):9291. doi: 10.1038/s41598-019-45743-6. - DOI - PMC - PubMed
    1. Lebek S, et al. CRISPR-Cas9 base editing of pathogenic CaMKIIδ improves cardiac function in a humanized mouse model. J Clin Invest. 2024;134(1):e175164. doi: 10.1172/JCI175164. - DOI - PMC - PubMed
    1. Gaudelli NM, et al. Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage. Nature. 2017;551(7681):464–471. doi: 10.1038/nature24644. - DOI - PMC - PubMed

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