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. 2024:2742:151-164.
doi: 10.1007/978-1-0716-3561-2_12.

Development of Organoids to Study Infectious Host Interactions

Aparajita Lahree  1 , Leona Gilbert  2
Affiliations

Development of Organoids to Study Infectious Host Interactions

Aparajita Lahree et al. Methods Mol Biol. 2024.

Abstract

Emerging organoid research is paving way for studies in infectious diseases. Described here is a technique for the generation of stem-cell derived organoids for human small intestine and lung together with methods to infect such organoids with a mock pathogen (Cryptosporidium parvum). Such systems are amenable to imaging and processing for molecular biological analyses. It is the intent of this chapter to provide a simple, routine organoid procedure so that in vitro studies with Borrelia such as cell invasion and dissemination can be conducted.

Keywords: 3-D cultures; Borrelia; Infectious disease; Lyme disease; Organoid.

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References

    1. Berglund J, Eitrem R, Ornstein K et al (1995) An epidemiologic study of Lyme disease in southern Sweden. N Engl J Med 333:1319–1327. https://doi.org/10.1056/NEJM199511163332004 - DOI - PubMed
    1. Ursinus J, Vrijmoeth HD, Harms MG et al (2021) Prevalence of persistent symptoms after treatment for Lyme Borreliosis: a prospective observational cohort study. Lancet Reg Health Eur 6:100142. https://doi.org/10.1016/j.lanepe.2021.100142 - DOI - PubMed - PMC
    1. Bechtold KT, Rebman AW, Crowder LA et al (2017) Standardized symptom measurement of individuals with early Lyme disease over time. Arch Clin Neuropsychol 32(2):129–141. https://doi.org/10.1093/arclin/acw098 - DOI - PubMed
    1. Wormser GP, McKenna D, Karmen CL et al (2020) Prospective evaluation of the frequency and severity of symptoms in Lyme disease patients with erythema migrans compared with matched controls at baseline, 6 months, and 12 months. Clin Infect Dis 71(12):3118–3124. https://doi.org/10.1093/cid/ciz1215 - DOI - PubMed - PMC
    1. Barthold SW, Beck DS, Hansen GM et al (1990) Lyme Borreliosis in selected strains and ages of laboratory mice. J Infect Dis 162(1):133–138. https://doi.org/10.1093/infdis/162.1.133