doi: 10.1007/BF00260401.
A hydrogen bond study in tobacco mosaic virus using Moessbauer spectroscopy
- PMID: 3816698
- DOI: 10.1007/BF00260401
Item in Clipboard
A hydrogen bond study in tobacco mosaic virus using Moessbauer spectroscopy
Eur Biophys J.
1986.
Abstract
The Moessbauer method was applied to obtain information on a suggested hydrogen bond in tobacco mosaic virus (TMV), between the hydroxyl group of Tyr 139 and a carboxyl oxygen of Glu 22 in a neighbouring subunit. Spectra of 129I were taken of 3,5-di-iodo-L-tyrosine as a free amino acid and in situ in TMV. The increase of the pK value of 3,5-di-iodo-L-tyrosine by 0.8 units at position 139 in TMV compared to the free value is a strong argument in favour of the existence of a hydrogen bond via the relevant hydroxyl group. The reported study demonstrates the surprising sensitivity of the observable Moessbauer parameters to details of the electronic configuration in the neighbourhood of the probe nucleus.
Similar articles
-
Tobacco mosaic virus reconstitution in the presence of 8 S TMV-protein component.FEBS Lett. 1974 Apr 15;41(1):25-9. doi: 10.1016/0014-5793(74)80945-2. FEBS Lett. 1974. PMID: 4855216 No abstract available.
-
Reinvestigation of intracellular localization of the 30K protein in tobacco protoplasts infected with tobacco mosaic virus RNA.Virology. 1992 Apr;187(2):809-13. doi: 10.1016/0042-6822(92)90484-7. Virology. 1992. PMID: 1546469
-
Studies on the mechanism of assembly of tobacco mosaic virus.Biophys J. 1980 Oct;32(1):313-29. doi: 10.1016/S0006-3495(80)84959-9. Biophys J. 1980. PMID: 7248451 Free PMC article.
-
Entropy-driven processes in biology.Mol Biol Biochem Biophys. 1975;(20):1-264. doi: 10.1007/978-3-642-80869-2. Mol Biol Biochem Biophys. 1975. PMID: 785232 Review. No abstract available.
-
Protein-RNA interactions during TMV assembly.J Supramol Struct. 1979;12(3):305-20. doi: 10.1002/jss.400120304. J Supramol Struct. 1979. PMID: 397974 Review.