Growth performance, histological and physiological responses of heat-stressed broilers in response to short periods of incubation during egg storage and thermal conditioning

Abstract

The short periods of incubation during egg storage (SPIDES) method enhances the quality of chicks and improves hatching rates. Additionally, embryonic thermal conditioning (TC) is a technique used to enhance thermotolerance in birds. Previous studies have evaluated the effects of SPIDES and embryonic TC separately. Yet, our hypothesis postulated that a synergistic effect could be achieved by integrating TC and SPIDES, thereby enhancing the broilers' resilience to thermal stress. We conducted an experiment involving 800 Ross broiler eggs, divided into two groups. The first group, referred to as S0, was maintained under standard storage room conditions and acted as our control group. The second group, known as S1, underwent a process called SPIDES for 5 h at a temperature of 37.8 ± 0.1 °C, on three occasions: days 5, 10, and 15 following egg collection. Upon reaching the 14th day of incubation (DOI), each of these primary groups was randomly subdivided into two equal subgroups. The control subgroup, designated as TC0, remained in the usual incubation conditions. Meanwhile, the other subgroup, TC1, was subjected to prenatal heat conditioning at a temperature of 39.5 ± 0.1 °C for 6 h per day, commencing on the 14th embryonic day (E) and extending until the 18th embryonic day (E). This experimental setup resulted in four distinct experimental subgroups: S0TC0, S1TC0, S0TC1, and S1TC1. The findings indicated that the combined application of SPIDES and TC had a significant positive effect on chick performance after hatching. Specifically, the (S1TC1) group exhibited the heaviest live body weight (LBW) and body weight gain (BWG) at the marketing age in comparison to the other groups. Furthermore, both SPIDES and TC had a positive influence on the relative weights of breast muscles and their histological measurements. The (S1TC1) group displayed significantly higher values in terms of the relative weight of breast muscles and the number of myocytes. In conclusion, SPIDES and TC have beneficial effects on pre- and post-hatch characteristics of broiler chicks up until the marketing age. Additionally, TC techniques improve chick performance, particularly under conditions of heat stress, and enhance the yield of breast muscle in later stages of life.

Figure 1

Transverse section through pectoralis breast muscle from birds of different treatments at 35 DOA. F fascicles, E endomysium, M myocytes, P perimysium (H & E ×100). S₀TC₀ = control no SPIDES & incubation under normal condition, S₀TC₁ control no SPIDES & prenatal thermal conditioning, S₁TC₀ SPIDES & incubation under normal condition, S₁TC₁ SPIDES & prenatal thermal conditioning, TC prenatal thermal conditioning, SPIDES short period of incubation during egg storage period.