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. 2023;14(11):595-599.
doi: 10.30466/vrf.2023.1998950.3826. Epub 2023 Nov 15.

Prevalence and genotypes of Giardia duodenalis in shelter dogs of southeastern Türkiye

Burçak Aslan Çelik  1 , Özgür Yaşar Çelik  2 , Akın Koçhan  3 , Adnan Ayan  4 , Özlem Orunç Kılınç  5 , Gürkan Akyıldız  6 , Kıvanç İrak  7 , Özge Oktay Ayan  8 , Kerem Ercan  2
Affiliations

Prevalence and genotypes of Giardia duodenalis in shelter dogs of southeastern Türkiye

Burçak Aslan Çelik et al. Vet Res Forum. 2023.

Abstract

Giardia duodenalis is a protozoan parasite found in humans and several mammals. This parasite spreads worldwide and is generally recognized as a zoonotic agent being reported to be one of the most common causes of diarrhea in humans and animals. In this study, it was aimed to determine the prevalence and genotypes of G. duodenalis in shelter dogs in Diyarbakır province being located in the southeastern Anatolia region of Türkiye. Native-Lugol method and nested polymerase chain reaction analyses of 100 fecal samples showed a prevalence of 3.00 and 4.00%, respectively. The prevalence was higher in females and in those younger than 1 year. Sequence analysis revealed the presence of zoonotic assemblage B, assemblage D and assemblage E. The detection of zoonotic assemblage B in this study suggests that dogs may be a reservoir for human giardiasis. Further molecular research is needed to determine the genotype diversity of Giardia as well as its possible role in the transmission of this parasite to humans.

Keywords: Giardia duodenalis; Nested polymerase chain reaction; Shelter dog; Türkiye.

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Conflict of interest statement

The authors state no conflict of interest.

Figures

Fig. 1
Fig. 1
Photomicrograph of the Giardia duodenalis cyst (arrow), (bar = 50 µm).
Fig. 2
Fig. 2
Polymerase chain reaction products of Giardia duodenalis. Lane M: DNA marker (511 bp); Lane N: Negative control; Lane P: Positive control; Lanes 36 - 39: G. duodenalis
Fig. 3
Fig. 3
Phylogenetic relationships of Giardia duodenalis isolates using maximum likelihood method analysis based on β-giardin gene region. Numbers at the nodes represent the bootstrap values (1,000 bootstrap). Giardia psitacci and Giardia muris were used as outgroup
See this image and copyright information in PMC

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