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. 2023 Dec 6;15(12):e50055.
doi: 10.7759/cureus.50055. eCollection 2023 Dec.

Antimicrobial Effectiveness Testing of Resorbable Electrospun Fiber Matrix per United States Pharmacopeia (USP) <51>

Emily Sallade  1 , Derayvia Grimes  1 , Lily Jeng  2 , Matthew R MacEwan  2
Affiliations

Antimicrobial Effectiveness Testing of Resorbable Electrospun Fiber Matrix per United States Pharmacopeia (USP) <51>

Emily Sallade et al. Cureus. .

Abstract

Contamination of surgical, traumatic, and chronic wounds with microorganisms presents a challenge to successful wound healing. In the present in vitro study, a synthetic electrospun fiber matrix (SEFM) cleared for use in the management of chronic, surgical, and traumatic wounds underwent USP (United States Pharmacopeia) <51> Antimicrobial Effectiveness Testing to determine its in vitro effectiveness against various microorganisms commonly found in non-healing wounds. The SEFM was tested in both sheet (s-SEFM) and micronized form (m-SEFM) against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Aspergillus brasiliensis, Candida albicans, Proteus mirabilis, and Enterococcus faecalis. Testing was performed per the USP <51> standard on days 7, 14, and 28. Both the s-SEFM and m-SEFM met the USP <51> acceptance criteria for all microorganisms. The results obtained for s-SEFM demonstrated >1-log10 reduction against E. coli, S. aureus, P. aeruginosa, P. mirabilis, E. faecalis, and C. albicans at day 7; >3-log10 reduction with no detection of these microbes at days 14 and 28, and no increase from initial inoculum at days 7, 14, and 28 against A. brasiliensis. The results obtained for m-SEFM demonstrated >3-log10 reduction with no detectable microorganisms at day 7. The results observed in this study indicate that the SEFM is effective in vitro at inhibiting bacterial and fungal growth and colonization per USP <51> testing.

Keywords: contaminated wound; contamination; electrospinning; extracellular matrix; non-healing wound; surgical wound; synthetic electrospun fiber matrix; traumatic wound; usp <51> antimicrobial effectiveness testing; wound healing.

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Conflict of interest statement

Matthew MacEwan is the patent owner of the Restrata matrix

Figures

Figure 1
Figure 1. Frequently occurring microorganisms present in wounds.
This figure is an original work of the authors.
Figure 2
Figure 2. Calculation used to determine microorganism concentration at enumeration steps
CFU: Colony forming units.
Figure 3
Figure 3. The calculation used to determine the difference of each test microorganism at the enumeration compared to the initial counts
CFU: Colony forming units.
Figure 4
Figure 4. The calculation used to determine the percent recovery of each microorganism for Neutralization Verification
See this image and copyright information in PMC

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