The complete mitochondrial genome of Semiothisa cinerearia Bremer & Grey 1853 (Lepidoptera: Geometridae: Ennominae) and its phylogenetic analysis

Abstract

Semiothisa cinerearia Bremer & Grey, 1853 belongs to the lepidopteran family Geometridae, subfamily Ennominae. We sequenced the complete mitochondrial genome of S. cinerearia by PCR and Sanger sequencing method. The mitochondrial genome of S. cinerearia is 15,523 bp in length and contains a typical set of 37 genes with 'MIQ' type gene arrangement and a 394 bp AT-rich regions. Except for cox1 using CGA as initiation codon, all protein-coding genes (PCGs) start with ATN codons and except for nad2 and nad4l using TAG as termination codon, all PCGs terminated with TAA codon. A phylogenetic tree including 39 genus of subfamily Ennominae was first reconstructed based on the mitochondrial genome sequences with nucleotide substitution model GTR + G + I, which showed that the genera Amraica, Jankowskia, and Ectropis are not monophyletic and S. cinerearia and Macaria notata are classified together.

Keywords: Ennominae; Geometridae; Semiothisa cinerearia; mitochondrial genome; phylogeny.

Figure 1.

Specimen of S. cinerearia was collected from the Xiangshan Mountain, Huaibei City, China. Photographed by Jun Li on 18 March 2023.

Figure 2.

Complete mitochondrial genome map of S. cinerearia with 13 protein coding genes, 22 tRNAs, 2 rRNAs, and an AT-rich region. Outside and inner circles represent the J- and M-strand respectively. Bold lines in circles represent strands in which the genes lie. (Green: tRNAs; yellow: PCGs; greenish yellow: rRNAs; grey: overlaps; white: interval spaces).

Figure 3.

The Bayesian inference (BI) phylogenetic tree was reconstructed based on published mitochondrial genome sequences of subfamily Ennominae and two species of subfamily Geometrinae were used as outgroup. GenBank accession numbers were indicated before the species names. Numbers at the nodes indicated Bayesian posterior probability values.