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. 1987 Feb;25(2):222-5.
doi: 10.1128/jcm.25.2.222-225.1987.

Production and purification of Streptococcus pneumoniae hemolysin (pneumolysin)

K Kanclerski, R Möllby

Production and purification of Streptococcus pneumoniae hemolysin (pneumolysin)

K Kanclerski et al. J Clin Microbiol. 1987 Feb.

Abstract

Pneumolysin was found to be produced by 112 of 113 clinical isolates of Streptococcus pneumoniae and to be an intracellular hemolysin. A 10-liter-scale fermentor production and purification procedure was developed for this hemolysin. The culture was concentrated by filtration 10 times before centrifugation. The cellular content was purified by ion-exchange chromatography, covalent thiopropyl gel chromatography, and gel filtration. One batch operation resulted in 6 mg of highly purified pneumolysin, with a yield of 66% and a specific activity of 1,400,000 hemolytic units per mg. The pneumolysin had a molecular weight of 53,000 and an isoelectric point of 5.2. The purification method developed will be of value in future studies on this hemolysin.

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References

    1. Infect Immun. 1982 Aug;37(2):837-9 - PubMed
    1. Am J Ophthalmol. 1971 Jul 30;72(1):175-80 - PubMed
    1. Infect Immun. 1984 Mar;43(3):1085-7 - PubMed
    1. Infect Immun. 1981 Oct;34(1):171-6 - PubMed
    1. J Bacteriol. 1942 May;43(5):607-27 - PubMed

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