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. 2021 Jul;4(3):229-239.
doi: 10.26655/AJNANOMAT.2021.3.5. Epub 2021 May 27.

Toxicity testing of indocyanine green and fluorodeoxyglucose conjugated iron oxide nanoparticles with and without exposure to a magnetic field

Perihan Unak  1 , Rachel Hepton  2 , Max Harper  3 , Volkan Yasakci  1 , Gillian Pearce  3 , Steve Russell  2 , Omer Aras  4 , Oguz Akin  4 , Julian Wong  5
Affiliations

Toxicity testing of indocyanine green and fluorodeoxyglucose conjugated iron oxide nanoparticles with and without exposure to a magnetic field

Perihan Unak et al. Asian J Nanosci Mater. 2021 Jul.

Abstract

Iron nanoparticles (MNPs) are known to induce membrane damage and apoptosis of cancer cells. In our study we determined whether FDG coupled with iron oxide magnetic nanoparticles can exert the same destructive effect on cancer cells. This research study presents data involving NIC-H727 human lung, bronchus epithelial cells exposed to conjugated fluorodeoxyglucose conjugated with iron-oxide magnetic nanoparticles and indocyanine green (ICG) dye (FDG-MNP-ICG), with and without the application of a magnetic field. Cell viability inferred from MTT assay revealed that FDG-MNPs had no significant toxicity towards noncancerous NIC-H727 human lung, bronchus epithelial cells. However, percentage cell death was much higher using a magnetic field, for the concentration of FDG-MNP-ICC used in our experiments. Magnetic field was able to destroy cells containing MNPs, while MNPs alone had significantly lower effects. Additionally, MNPs alone in these low concentrations had less adverse effects on healthy (non-target) cells.

Keywords: Fluorodeoxyglucose; Indocyanine green; Iron oxide nanoparticles; Magnetic effect; NIC-H7272 cells.

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Conflict of interest statement

Disclosure Statement No potential conflict of interest was reported by the authors.

Figures

Figure 1.
Figure 1.
Dynamic light scattering spectra of the FDG-MNPs
Figure 2.
Figure 2.
SEM Image of FDG-MNPs
Figure 3.
Figure 3.
Fourier transform infrared spectra (FTIR) of FDG-MNPs
Figure 4.
Figure 4.
Toxicity test of FDG-MNP-ICG. (The table shows the concentrations and volumes for each well)
Figure 5.
Figure 5.
a) Dose-response curve of MNPs shown by the percentage cell death caused by five different concentrations and b) cytotoxicity plot ± SD of same agents on noncancerous cell NIC-H727 human lung, bronchus epithelial cells (EC50) over a period of three time points
See this image and copyright information in PMC

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