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. 2023 Dec;176(2):283-289.
doi: 10.1007/s10517-024-06009-y. Epub 2024 Jan 9.

Biogenesis of Mitochondria in Multipotent Mesenchymal Stromal Cells in Patients with Acute Leukemia

A V Sadovskaya  1 , N A Petinati  2 , N V Sats  2 , N I Drize  2 , A N Vasil'eva  2 , O A Aleshina  2 , E N Parovichnikova  2
Affiliations

Biogenesis of Mitochondria in Multipotent Mesenchymal Stromal Cells in Patients with Acute Leukemia

A V Sadovskaya et al. Bull Exp Biol Med. 2023 Dec.

Abstract

In patients with acute leukemia, not only normal hematopoiesis, but also bone marrow stromal microenvironment is damaged. Multipotent mesenchymal stromal cells (MSC) are essential for the formation and function of the stromal microenvironment. Analysis of changes in MSC is important for the development of new approaches to leukemia therapy. The metabolism of mitochondria in MSC, relative content of mitochondrial DNA, and expression levels of genes encoding PGC-1α and Nrf2 proteins, important regulators of biogenesis, were studied using real-time PCR. Relative content of mitochondrial DNA does not change in MSC of acute leukemia patients at the onset of disease or in remission. Relative expression level of the gene encoding PGC-1α protein in MSC does not change significantly. However, relative expression level of the gene encoding Nrf2, an important antioxidant activity regulator, insignificantly decreases in patients at the onset of acute leukemia, and this decrease becomes significant upon reaching remission.

Keywords: acute leukemia; gene expression; mitochondrial DNA; multipotent mesenchymal stromal cells.

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References

    1. Morrison SJ, Scadden DT. The bone marrow niche for haematopoietic stem cells. Nature. 2014;505:327-334. doi: https://doi.org/10.1038/nature12984 - DOI - PubMed - PMC
    1. Phinney DG, Di Giuseppe M, Njah J, Sala E, Shiva S, St Croix CM, Stolz DB, Watkins SC, Di YP, Leikauf GD, Kolls J, Riches DW, Deiuliis G, Kaminski N, Boregowda SV, McKenna DH, Ortiz LA. Mesenchymal stem cells use extracellular vesicles to outsource mitophagy and shuttle microRNAs. Nat. Commun. 2015;6:8472. doi: https://doi.org/10.1038/ncomms9472 - DOI - PubMed
    1. Batsivari A, Grey W, Bonnet D. Understanding of the crosstalk between normal residual hematopoietic stem cells and the leukemic niche in acute myeloid leukemia. Exp. Hematol. 2021;95:23-30. doi: https://doi.org/10.1016/j.exphem.2021.01.004 - DOI - PubMed
    1. Baryawno N, Severe N, Scadden DT. Hematopoiesis: reconciling historic controversies about the niche. Cell Stem Cell. 2017;20(5):590-592. doi: https://doi.org/10.1016/j.stem.2017.03.025 - DOI - PubMed
    1. Lyamzaev KG, Zinovkin RA, Chernyak BV. Extrusion of mitochondria: Garbage clearance or cell-cell communication signals? J. Cell. Physiol. 2022;237(5):2345-2356. doi: https://doi.org/10.1002/jcp.30711 - DOI - PubMed

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