ELISA for measurement of secretory IgA distinct from monomeric IgA

Abstract

A micro enzyme-linked immunoassay (ELISA) is described for the quantitation of secretory IgA as distinct from monomeric IgA. The assay is sensitive (linear down to 30 ng/ml) and reproducible (inter-assay variation: 17.0%; intra-assay variation: 11.5%). The assay has the further advantages of rapidity, the ability to handle large numbers of samples, and uses commercially available reagents throughout. Minimal interference from a large (greater than 25-fold) excess of monomeric IgA was observed. The results obtained for serum secretory IgA concentrations by this method correlated well with those reported by other workers for normal control patients, patients with IgA deficiency and patients with liver disease.