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. 2024 Jan 15:98:117580.
doi: 10.1016/j.bmc.2023.117580. Epub 2023 Dec 25.

A new small molecule DoNA binding to CAG repeat RNA

Qingwen Chen  1 , Takeshi Yamada  2 , Koichi Miyagawa  3 , Asako Murata  4 , Mitsuo Shoji  3 , Kazuhiko Nakatani  5
Affiliations

A new small molecule DoNA binding to CAG repeat RNA

Qingwen Chen et al. Bioorg Med Chem. .

Abstract

We here report a new molecule DoNA binding to a CAG repeat RNA. DoNA is a dimer of the NA molecule that we previously reported. NA binds with high affinity to a CAG repeat DNA but not significantly to a CAG repeat RNA. Binding analyses using SPR and CSI-TOF MS indicated a significant increase in the affinity of DoNA to a single stranded CAG repeat RNA compared to NA. Systematic investigation of the RNA motifs bound by DoNA using hairpin RNA models revealed that DoNA binds to the CAG units at overhang and terminal positions, and notably, it binds to the structurally flexible internal and hairpin loop region.

Keywords: CAG repeats; DoNA; RNA binding small molecule.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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